Siyuan Tang1,2, Bailong Liu1,3, Jiaqi Liu1, Jian Wang1, Ya Wang1. 1. a Department of Radiation Oncology , Emory University School of Medicine, Winship Cancer Institute of Emory University , Atlanta , GA , USA. 2. b Department of Oncology , Xiangya Hospital, Central South University , Changsha , China. 3. c Department of Radiation Oncology , The First Norman Bethune Hospital of Jilin University , Changchun , China.
Abstract
PURPOSE: To study whether miR-21, an oncogene associated with lung tumorigenesis, affects immune response. MATERIAL AND METHODS: Cancer immune-related 786 mRNA expression was compared in lung tissue from wild-type and miR-21 knock-in mice using NanoString technology. The significantly changed genes were verified using real-time PCR. E-Selectin (Sele) was subsequently identified for further examination using immunohistochemistry (IHC) and Western blot in the same lung tissue. The mouse Sele 3'untranslated region (3'-UTR) was searched to identify a miR-21 matching sequence. The Sele level in miR-21 mimic transfected mouse lung bronchial epithelial (LBE) cells was examined. RESULTS: We unexpectedly found that the Sele mRNA level significantly increased but the protein level significantly decreased in the lung tissue of miR-21 knock-in mice compared to the mRNA/protein levels in the lung tissue of wild-type mice. The mouse Sele 3'-UTR contains the key sequence that can be targeted by miR-21. The Sele levels decreased in mouse LBE cells after miR-21 mimic transfection. CONCLUSION: Sele is a potential miR-21 target. The opposing Sele levels at mRNA and protein suggest a feedback-regulation from protein to mRNA. The feedback-regulation in miR-21-suppressed gene expression indicates that we should carefully evaluate any data from mRNA array since they may not reflect real protein expression status.
PURPOSE: To study whether miR-21, an oncogene associated with lung tumorigenesis, affects immune response. MATERIAL AND METHODS:Cancer immune-related 786 mRNA expression was compared in lung tissue from wild-type and miR-21 knock-in mice using NanoString technology. The significantly changed genes were verified using real-time PCR. E-Selectin (Sele) was subsequently identified for further examination using immunohistochemistry (IHC) and Western blot in the same lung tissue. The mouseSele 3'untranslated region (3'-UTR) was searched to identify a miR-21 matching sequence. The Sele level in miR-21 mimic transfected mouse lung bronchial epithelial (LBE) cells was examined. RESULTS: We unexpectedly found that the Sele mRNA level significantly increased but the protein level significantly decreased in the lung tissue of miR-21 knock-in mice compared to the mRNA/protein levels in the lung tissue of wild-type mice. The mouseSele 3'-UTR contains the key sequence that can be targeted by miR-21. The Sele levels decreased in mouse LBE cells after miR-21 mimic transfection. CONCLUSION:Sele is a potential miR-21 target. The opposing Sele levels at mRNA and protein suggest a feedback-regulation from protein to mRNA. The feedback-regulation in miR-21-suppressed gene expression indicates that we should carefully evaluate any data from mRNA array since they may not reflect real protein expression status.
Authors: B J Graves; R L Crowther; C Chandran; J M Rumberger; S Li; K S Huang; D H Presky; P C Familletti; B A Wolitzky; D K Burns Journal: Nature Date: 1994-02-10 Impact factor: 49.962
Authors: Mark E Hatley; David M Patrick; Matthew R Garcia; James A Richardson; Rhonda Bassel-Duby; Eva van Rooij; Eric N Olson Journal: Cancer Cell Date: 2010-09-14 Impact factor: 31.743