Literature DB >> 3063209

Cultivation of cottontail rabbit epidermal (Sf1Ep) cells on microcarrier beads and their use for suspension cultivation of Treponema pallidum subsp. pallidum.

B S Riley1, D L Cox.   

Abstract

In vitro propagation of Treponema pallidum can be achieved by cocultivation with Sf1Ep cells. This study had two objectives: (i) to achieve suspension cultivation of Sf1Ep cells and (ii) to develop procedures for achieving the replication of T. pallidum in those cell cultures. Seven suspension cultures of Sf1Ep cells yielded an average of 7.2 x 10(8) T. pallidum (36-fold increase) after 12 days.

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Year:  1988        PMID： 3063209      PMCID： PMC204387          DOI： 10.1128/aem.54.11.2862-2865.1988

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Epidermal cell culture using Sephadex beads coated with denatured collagen (cytodex 3).

Authors:  H Katayama; S Itami; H Koizumi; M Tsutsui
Journal:  J Invest Dermatol       Date:  1987-01       Impact factor: 8.551

2.  Cell growth optimization in microcarrier culture.

Authors:  B Mered; P Albrecht; H E Hopps
Journal:  In Vitro       Date:  1980-10

3.  Production of cell-derived products: virus and interferon.

Authors:  A J Sinskey; R J Fleischaker; M A Tyo; D J Giard; D I Wang
Journal:  Ann N Y Acad Sci       Date:  1981       Impact factor: 5.691

4.  Propagation of poliovirus in microcarrier cultures of three monkey kidney cell lines.

Authors:  B Mered; P Albrecht; H E Hopps; J C Petricciani; J Salk
Journal:  J Biol Stand       Date:  1981

5.  Optimizing culture conditions for the production of animal cells in microcarrier culture.

Authors:  J M Clark; M D Hirtenstein
Journal:  Ann N Y Acad Sci       Date:  1981       Impact factor: 5.691

6.  In vitro cultivation of Treponema pallidum: independent confirmation.

Authors:  S J Norris
Journal:  Infect Immun       Date:  1982-04       Impact factor: 3.441

7.  Prolonged survival of virulent Treponema pallidum (Nichols strain) in cell-free and tissue culture systems.

Authors:  A H Fieldsteel; F A Becker; J G Stout
Journal:  Infect Immun       Date:  1977-10       Impact factor: 3.441

8.  Cultivation of virulent Treponema pallidum in tissue culture.

Authors:  A H Fieldsteel; D L Cox; R A Moeckli
Journal:  Infect Immun       Date:  1981-05       Impact factor: 3.441

9.  Culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers.

Authors:  A J Hayle
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

10.  Further studies on replication of virulent Treponema pallidum in tissue cultures of Sf1Ep cells.

Authors:  A H Fieldsteel; D L Cox; R A Moeckli
Journal:  Infect Immun       Date:  1982-02       Impact factor: 3.441
  10 in total
  2 in total

1.  Evidence that TP_0144 of Treponema pallidum is a thiamine-binding protein.

Authors:  Jiang Bian; Youbin Tu; Song-Mei Wang; Xuan-Yi Wang; Chunhao Li
Journal:  J Bacteriol       Date:  2015-01-20       Impact factor: 3.490

2.  New Tools for Syphilis Research.

Authors:  Sheila A Lukehart
Journal:  MBio       Date:  2018-07-31       Impact factor: 7.867
  2 in total

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