Literature DB >> 30630374

In vitro gene expression and detergent-free reconstitution of active proteorhodopsin in lipid vesicles.

Giorgio Fracasso1, Yvonne Körner1, David Thomas T Gonzales1, T-Y Dora Tang1.   

Abstract

IMPACT STATEMENT: Our results offer the potential for straightforward, additive-free, and molecularly simple routes to building complex bioreactors based on in vitro transcription-translation systems and lipid vesicles.

Keywords:  Lipids synthetic biology; artificial cells; cell-free protein expression; protein reconstitution; proteorhodopsin

Year:  2019        PMID： 30630374      PMCID： PMC6435890          DOI： 10.1177/1535370218820290

Source DB:  PubMed          Journal:  Exp Biol Med (Maywood)        ISSN： 1535-3699


  35 in total

Review 1.  Artificial cells: prospects for biotechnology.

Authors:  Andrew Pohorille; David Deamer
Journal:  Trends Biotechnol       Date:  2002-03       Impact factor: 19.536

2.  Proteorhodopsin is a light-driven proton pump with variable vectoriality.

Authors:  Thomas Friedrich; Sven Geibel; Rolf Kalmbach; Igor Chizhov; Kenichi Ataka; Joachim Heberle; Martin Engelhard; Ernst Bamberg
Journal:  J Mol Biol       Date:  2002-08-30       Impact factor: 5.469

3.  A vesicle bioreactor as a step toward an artificial cell assembly.

Authors:  Vincent Noireaux; Albert Libchaber
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-10       Impact factor: 11.205

4.  Artificial organelle: ATP synthesis from cellular mimetic polymersomes.

Authors:  Hyo-Jick Choi; Carlo D Montemagno
Journal:  Nano Lett       Date:  2005-12       Impact factor: 11.189

5.  Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host.

Authors:  A Martinez; A S Bradley; J R Waldbauer; R E Summons; E F DeLong
Journal:  Proc Natl Acad Sci U S A       Date:  2007-03-19       Impact factor: 11.205

6.  Optimized in vitro and in vivo expression of proteorhodopsin: a seven-transmembrane proton pump.

Authors:  Pontus Gourdon; Anna Alfredsson; Anders Pedersen; Erik Malmerberg; Maria Nyblom; Mikael Widell; Ronnie Berntsson; Jarone Pinhassi; Marc Braiman; Orjan Hansson; Nicklas Bonander; Göran Karlsson; Richard Neutze
Journal:  Protein Expr Purif       Date:  2007-11-20       Impact factor: 1.650

7.  Folding and assembly of proteorhodopsin.

Authors:  Adriana L Klyszejko; Sarika Shastri; Stefania A Mari; Helmut Grubmüller; Daniel J Muller; Clemens Glaubitz
Journal:  J Mol Biol       Date:  2007-11-19       Impact factor: 5.469

8.  Rapid protein-folding assay using green fluorescent protein.

Authors:  G S Waldo; B M Standish; J Berendzen; T C Terwilliger
Journal:  Nat Biotechnol       Date:  1999-07       Impact factor: 54.908

9.  Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex.

Authors:  Haoyong Wang; Shaorong Chong
Journal:  Proc Natl Acad Sci U S A       Date:  2003-01-06       Impact factor: 11.205

10.  Investigation into the use of C- and N-terminal GFP fusion proteins for subcellular localization studies using reverse transfection microarrays.

Authors:  Ella Palmer; Tom Freeman
Journal:  Comp Funct Genomics       Date:  2004
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  2 in total

1.  Community building in synthetic biology.

Authors:  Oscar Ces; Yuval Elani
Journal:  Exp Biol Med (Maywood)       Date:  2019-03

2.  Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion.

Authors:  Tony Köhler; Thomas Heida; Sandra Hoefgen; Niclas Weigel; Vito Valiante; Julian Thiele
Journal:  RSC Adv       Date:  2020-11-09       Impact factor: 4.036
  2 in total

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