Pralhad Namdev Joshi1, Vishal Rai1. 1. Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhauri, Bhopal, MP 462 066, India. vrai@iiserb.ac.in.
Abstract
A precision methodology distinguishes one His from all the nucleophilic residues and its multiple copies. An easy-to-operate C-N bond formation labels diverse proteins without adversely affecting their structure and function. The late-stage transformation allows installation of distinct probes. The chemically triggered reversibility enables traceless metal-free purification of proteins with a His-tag.
A precision methodology distinguishes one His from all the nucleophilic residues and its multiple copies. An easy-to-operate C-n class="Chemical">N bond formation labels diverse proteins without adversely affecting their structure and function. The late-stage transformation allows installation of distinct probes. The chemically triggered reversibility enables traceless metal-free purification of proteins with a His-tag.