Literature DB >> 3061801

The binding site for ribosomal protein L2 within 23S ribosomal RNA of Escherichia coli.

A A Beauclerk1, E Cundliffe.   

Abstract

Ribosomal protein L2 from Escherichia coli binds to and protects from nuclease digestion a substantial portion of 'domain IV' of 23S rRNA. In particular, oligonucleotides derived from the sequence 1757-1935 were isolated and shown to rebind specifically to protein L2 in vitro. Other L2-protected oligonucleotides, also derived from domain IV (i.e. from residues 1955-2010) did not rebind to protein L2 in vitro nor did others derived from domain I. Given that protein L2 is widely believed to be located in the peptidyl transferase centre of the 50S ribosomal subunit, these data suggest that domain IV of 23S rRNA is also present in that active site of the ribosomal enzyme.

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Year:  1988        PMID： 3061801      PMCID： PMC454861          DOI： 10.1002/j.1460-2075.1988.tb03236.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  29 in total

1.  Cooperative interactions among protein and RNA components of the 50S ribosomal subunit of Escherichia coli.

Authors:  P Spierer; C C Wang; T L Marsh; R A Zimmermann
Journal:  Nucleic Acids Res       Date:  1979-04       Impact factor: 16.971

2.  Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis.

Authors:  R E Lockard; B Alzner-Deweerd; J E Heckman; J MacGee; M W Tabor; U L RajBhandary
Journal:  Nucleic Acids Res       Date:  1978-01       Impact factor: 16.971

3.  Purification of ribosomal proteins from Escherichia coli under nondenaturing conditions.

Authors:  J Dijk; J Littlechild
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

4.  Photoinduced affinity labeling of the Escherichia coli ribosome puromycin site.

Authors:  E N Jaynes; P G Grant; G Giangrande; R Wieder; B S Cooperman
Journal:  Biochemistry       Date:  1978-02-21       Impact factor: 3.162

5.  Binding sites of ribosomal proteins on two specific fragments derived from Escherichia coli 50 S ribosomes.

Authors:  B Allet; P F Spahr
Journal:  Eur J Biochem       Date:  1971-03-11

6.  Specific labeling of 3' termini of RNA with T4 RNA ligase.

Authors:  T E England; A G Bruce; O C Uhlenbeck
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  RNA-protein interactions in the ribosome. Binding of proteins L1, L3, L6, L13 and L23 to specific fragments of the 23S RNA.

Authors:  P Spierer; R A Zimmermann
Journal:  FEBS Lett       Date:  1976-09-15       Impact factor: 4.124

8.  RNA-protein interactions in the ribosome. Binding of 50-S-subunit proteins to 5' and 3' terminal segments of the 23-S RNA.

Authors:  P Spierer; R A Zimmerman; G A Mackie
Journal:  Eur J Biochem       Date:  1975-04-01

9.  [3H]-p-azidopuromycin photoaffinity labeling of Escherichia coli ribosomes: evidence for site-specific interaction at U-2504 and G-2502 in domain V of 23S ribosomal RNA.

Authors:  C C Hall; D Johnson; B S Cooperman
Journal:  Biochemistry       Date:  1988-05-31       Impact factor: 3.162

10.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971
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  10 in total

1.  Selective isolation and detailed analysis of intra-RNA cross-links induced in the large ribosomal subunit of E. coli: a model for the tertiary structure of the tRNA binding domain in 23S RNA.

Authors:  P Mitchell; M Osswald; D Schueler; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

2.  Precise determination of RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli.

Authors:  B Thiede; H Urlaub; H Neubauer; G Grelle; B Wittmann-Liebold
Journal:  Biochem J       Date:  1998-08-15       Impact factor: 3.857

3.  Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies.

Authors:  M Uhlein; W Weglöhner; H Urlaub; B Wittmann-Liebold
Journal:  Biochem J       Date:  1998-04-15       Impact factor: 3.857

4.  Transfer RNA docking pair model in the ribosomal pre- and post-translocational states.

Authors:  K Nagano; N Nagano
Journal:  Nucleic Acids Res       Date:  1997-03-15       Impact factor: 16.971

5.  Probing the structure of mouse Ehrlich ascites cell 5.8S, 18S and 28S ribosomal RNA in situ.

Authors:  L Holmberg; Y Melander; O Nygård
Journal:  Nucleic Acids Res       Date:  1994-04-25       Impact factor: 16.971

6.  The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome.

Authors:  A Nakagawa; T Nakashima; M Taniguchi; H Hosaka; M Kimura; I Tanaka
Journal:  EMBO J       Date:  1999-03-15       Impact factor: 11.598

7.  Labeling the peptidyltransferase center of the Escherichia coli ribosome with photoreactive tRNA(Phe) derivatives containing azidoadenosine at the 3' end of the acceptor arm: a model of the tRNA-ribosome complex.

Authors:  J Wower; S S Hixson; R A Zimmermann
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

8.  Selection for intragenic suppressors of lethal 23S rRNA mutations in Escherichia coli identifies residues important for ribosome assembly and function.

Authors:  Michael O'Connor
Journal:  Mol Genet Genomics       Date:  2007-09-06       Impact factor: 3.291

9.  Cloning, sequencing and overexpression of the gene for prokaryotic factor EF-P involved in peptide bond synthesis.

Authors:  H Aoki; S L Adams; D G Chung; M Yaguchi; S E Chuang; M C Ganoza
Journal:  Nucleic Acids Res       Date:  1991-11-25       Impact factor: 16.971

10.  Role for the highly conserved region of domain IV of 23S-like rRNA in subunit-subunit interactions at the peptidyl transferase centre.

Authors:  I Leviev; S Levieva; R A Garrett
Journal:  Nucleic Acids Res       Date:  1995-05-11       Impact factor: 16.971
  10 in total

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