Catherine L Callahan1,2, Matthew R Bonner3, Jing Nie3, Youjin Wang3,4, Meng-Hua Tao5, Peter G Shields6, Catalin Marian6,7, Kevin H Eng8, Maurizio Trevisan9, Jo L Freudenheim3. 1. Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. Catherine.callahan@nih.gov. 2. Department of Epidemiology and Environmental Health, School of Public Health and Health Professions, University at Buffalo, Buffalo, NY, USA. Catherine.callahan@nih.gov. 3. Department of Epidemiology and Environmental Health, School of Public Health and Health Professions, University at Buffalo, Buffalo, NY, USA. 4. Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. 5. Department of Biostatistics and Epidemiology, University of North Texas Health Science Center, Fort Worth, TX, USA. 6. Division of Cancer Prevention and Control, College of Medicine and The Ohio State University Comprehensive Cancer Center, Columbus, OH, USA. 7. Department of Biochemistry and Pharmacology, University of Medicine and Pharmacy Timisoara, Timisoara, Romania. 8. Department of Biostatistics and Bioinformatics, Roswell Park Cancer Institute, Buffalo, NY, USA. 9. City College of New York, New York, NY, USA.
Abstract
PURPOSE: Tobacco smoke exposure has been associated with altered DNA methylation. However, there is a paucity of information regarding tobacco smoke exposure and DNA methylation of breast tumors. METHODS: We conducted a case-only analysis using breast tumor tissue from 493 postmenopausal and 225 premenopausal cases in the Western New York Exposures and Breast Cancer (WEB) study. Methylation of nine genes (SFN, SCGB3A1, RARB, GSTP1, CDKN2A, CCND2, BRCA1, FHIT, and SYK) was measured with pyrosequencing. Participants reported their secondhand smoke (SHS) and active smoking exposure for seven time periods. Unconditional logistic regression was used to estimate odds ratios (OR) of having methylation higher than the median. RESULTS: SHS exposure was associated with tumor DNA methylation among postmenopausal but not premenopausal women. Active smoking at certain ages was associated with increased methylation of GSTP1, FHIT, and CDKN2A and decreased methylation of SCGB3A1 and BRCA1 among both pre- and postmenopausal women. CONCLUSION: Exposure to tobacco smoke may contribute to breast carcinogenesis via alterations in DNA methylation. Further studies in a larger panel of genes are warranted.
PURPOSE: Tobacco smoke exposure has been associated with altered DNA methylation. However, there is a paucity of information regarding tobacco smoke exposure and DNA methylation of breast tumors. METHODS: We conducted a case-only analysis using breast tumor tissue from 493 postmenopausal and 225 premenopausal cases in the Western New York Exposures and Breast Cancer (WEB) study. Methylation of nine genes (SFN, SCGB3A1, RARB, GSTP1, CDKN2A, CCND2, BRCA1, FHIT, and SYK) was measured with pyrosequencing. Participants reported their secondhand smoke (SHS) and active smoking exposure for seven time periods. Unconditional logistic regression was used to estimate odds ratios (OR) of having methylation higher than the median. RESULTS: SHS exposure was associated with tumor DNA methylation among postmenopausal but not premenopausal women. Active smoking at certain ages was associated with increased methylation of GSTP1, FHIT, and CDKN2A and decreased methylation of SCGB3A1 and BRCA1 among both pre- and postmenopausal women. CONCLUSION: Exposure to tobacco smoke may contribute to breast carcinogenesis via alterations in DNA methylation. Further studies in a larger panel of genes are warranted.
Entities:
Keywords:
Breast cancer; DNA methylation; Epigenetics; Secondhand smoke; Tobacco
Authors: Matthew R Bonner; Jing Nie; Daikwon Han; John E Vena; Peter Rogerson; Paola Muti; Maurizio Trevisan; Stephen B Edge; Jo L Freudenheim Journal: Cancer Causes Control Date: 2005-08 Impact factor: 2.506
Authors: Francesco D'Agostini; Alberto Izzotti; Roumen Balansky; Nicola Zanesi; Carlo M Croce; Silvio De Flora Journal: Cancer Res Date: 2006-04-01 Impact factor: 12.701
Authors: Meng Hua Tao; Peter G Shields; Jing Nie; Amy Millen; Christine B Ambrosone; Stephen B Edge; Shiva S Krishnan; Catalin Marian; Bin Xie; Janet Winston; Dominica Vito; Maurizio Trevisan; Jo L Freudenheim Journal: Breast Cancer Res Treat Date: 2008-05-08 Impact factor: 4.872
Authors: Aslaug Aa Muggerud; Jo Anders Rønneberg; Fredrik Wärnberg; Johan Botling; Florence Busato; Jovana Jovanovic; Hiroko Solvang; Ida Bukholm; Anne-Lise Børresen-Dale; Vessela N Kristensen; Therese Sørlie; Jörg Tost Journal: Breast Cancer Res Date: 2010-01-07 Impact factor: 6.466
Authors: Paige M Hulls; Frank de Vocht; Yanchun Bao; Caroline L Relton; Richard M Martin; Rebecca C Richmond Journal: Environ Res Date: 2020-07-31 Impact factor: 6.498