| Literature DB >> 30616155 |
Zhichang Sun1, Xuerou Wang1, Zongwen Tang1, Qi Chen1, Xing Liu2.
Abstract
A biotin-streptavidin-amplified enzyme-linked immunosorbent assay using a biotinylated nanobody (BA-Nb ELISA) was developed to detect ochratoxin A (OTA) in cereal. The limit of detection (LOD) of the BA-Nb ELISA, which equals to 10% maximal inhibitory concentration, was 0.011 ng/mL for OTA in buffer, and the sensitivity was approximately improved by one order of magnitude compared with the traditional Nb ELISA (LOD = 0.112 ng/mL). Under optimal conditions, the developed assay could be accomplished in 40 min with maximal inhibitory concentration of 0.138 ng/mL and the linear detection range of 0.034-0.460 ng/mL. The average recovery rate of the BA-Nb ELISA ranged from 92.8% to 114%, and the relative standard deviation was in the range of 2.04-9.85%. The developed BA-Nb ELISA was validated by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the results indicated the reliability of BA-Nb ELISA for the detection of OTA in cereal.Entities:
Keywords: Biotin-streptavidin amplification; Cereal; Immunoassay; Nanobody; Ochratoxin A
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Year: 2019 PMID： 30616155 DOI： 10.1016/j.ecoenv.2018.12.103
Source DB: PubMed Journal: Ecotoxicol Environ Saf ISSN： 0147-6513 Impact factor: 6.291