Wejdene Mansour1,2, Raoudha Grami1, Nadia Jaidane1, Aziza Messaoudi1, Karama Charfi1, Lotfi Ben Romdhane3, Anis Bel Haj Khalifa4,5, Mohamed Khedher4,5, Noureddine Boujaafar1,5, Olfa Bouallègue1,2, Hedi Mammeri6,7,8. 1. 1 Research Unit Emergent Bacterial Resistance and Safety of Care UR12SP37, Laboratory of Microbiology, University Hospital Sahloul, Sousse, Tunisia. 2. 2 Faculty of Medicine Ibn Al Jazzar, Sousse, University of Sousse, Tunisia. 3. 3 Research Unit MARS (Modeling of Automated Reasoning Systems) University of Sousse, Sousse, Tunisia. 4. 4 Laboratory of Microbiology, University Hospital Tahar Sfar, Mahdia, Tunisia. 5. 5 Faculty of Pharmacy, Monastir, University of Monastir, Monastir, Tunisia. 6. 6 INSERM, IAME, UMR 1137, Paris, France. 7. 7 University Paris Diderot, IAME, UMR 1137, Sorbonne, Paris, France. 8. 8 Service of Bacteriology, University Hospital Bichat, Paris, France.
Abstract
Objectives: The whole-genome sequence (WGS) of Klebsiella pneumoniae KP3771 isolate was characterized. This strain was recovered from the urine sample of an 80-year-old man hospitalized in an intensive care unit of the University Hospital Tahar Sfar in Tunisia. Materials and Methods: WGS using a MiSeq platform was used. The assembled genome was subjected to several software analyses. Results: K. pneumoniae KP3771 was resistant to all antibiotics but colistin and tigecycline. WGS analysis found 18 transmissible genes encoding resistance markers, including blaNDM-1 and blaCTX-M-15 genes, which were carried by four plasmids belonging to the Inc Ib, IIk, and R groups. Three families of genes encoding virulence factors were detected, including adhesins (fimH, fimA, fimB, fimC, mrkD, Kpn, and ycfM), siderophores (enterobactin, aerobactin, and yersiniabactin siderophores), and protectin/invasin (traT). The strain was assigned to the sequence type 147. Conclusions: This study describes the genome of a carbapenemase-producing K. pneumoniae clinical isolate recovered in Tunisia. Bacteria WGS has become the reference technology to address epidemiological issues; this high level of information is particularly well suited to enrich epidemiological workflows' output.
Objectives: The whole-genome sequence (WGS) of Klebsiella pneumoniae KP3771 isolate was characterized. This strain was recovered from the urine sample of an 80-year-old man hospitalized in an intensive care unit of the University Hospital Tahar Sfar in Tunisia. Materials and Methods: WGS using a MiSeq platform was used. The assembled genome was subjected to several software analyses. Results:K. pneumoniae KP3771 was resistant to all antibiotics but colistin and tigecycline. WGS analysis found 18 transmissible genes encoding resistance markers, including blaNDM-1 and blaCTX-M-15 genes, which were carried by four plasmids belonging to the Inc Ib, IIk, and R groups. Three families of genes encoding virulence factors were detected, including adhesins (fimH, fimA, fimB, fimC, mrkD, Kpn, and ycfM), siderophores (enterobactin, aerobactin, and yersiniabactin siderophores), and protectin/invasin (traT). The strain was assigned to the sequence type 147. Conclusions: This study describes the genome of a carbapenemase-producing K. pneumoniae clinical isolate recovered in Tunisia. Bacteria WGS has become the reference technology to address epidemiological issues; this high level of information is particularly well suited to enrich epidemiological workflows' output.
Authors: Zena Lapp; Ryan Crawford; Arianna Miles-Jay; Ali Pirani; William E Trick; Robert A Weinstein; Mary K Hayden; Evan S Snitkin; Michael Y Lin Journal: Clin Infect Dis Date: 2021-10-20 Impact factor: 9.079