Yang Zheng1, Yiseng Hu2, Shuo Yan1, Hang Zhou1, Dunlun Song1, Meizhen Yin2, Jie Shen1. 1. Department of Entomology and MOA Key Laboratory for Monitory and Green Control of Crop Pest, China Agricultural University, Beijing, China. 2. State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, China.
Abstract
BACKGROUND: It is difficult to efficiently silence gene expression in some insects, probably because of the degradation of dsRNA by enzymes present in the gut and hemolymph post-oral feeding or body injecting of dsRNA. We previously developed a nanocarrier delivery system that can systemically deliver dsRNA into chewing mouthpart insects by oral feeding and efficiently silence gene expression. For the purpose of pest control in the field, there is a great demand to develop a spray method to apply dsRNA formulation. RESULTS: A formulation of the nanocarrier/dsRNA/detergent was developed and could be easily applied just by dropping it on the notum of the aphid. The formulation penetrated the body wall into the hemocoel and then spread into various tissues within 1 h. The delivered dsRNA efficiently silenced the target gene expression at a high knockdown effect (95.4%) and the aphid population was largely suppressed (80.5%). CONCLUSION: A novel dsRNA formulation was developed with the help of a nanocarrier and detergent that can quickly penetrate the insect body wall and efficiently silence gene expression. The formulation may provide a fast and easy tool for gene silence in some tough insects and for pest control in the field.
BACKGROUND: It is difficult to efficiently silence gene expression in some insects, probably because of the degradation of dsRNA by enzymes present in the gut and hemolymph post-oral feeding or body injecting of dsRNA. We previously developed a nanocarrier delivery system that can systemically deliver dsRNA into chewing mouthpart insects by oral feeding and efficiently silence gene expression. For the purpose of pest control in the field, there is a great demand to develop a spray method to apply dsRNA formulation. RESULTS: A formulation of the nanocarrier/dsRNA/detergent was developed and could be easily applied just by dropping it on the notum of the aphid. The formulation penetrated the body wall into the hemocoel and then spread into various tissues within 1 h. The delivered dsRNA efficiently silenced the target gene expression at a high knockdown effect (95.4%) and the aphid population was largely suppressed (80.5%). CONCLUSION: A novel dsRNA formulation was developed with the help of a nanocarrier and detergent that can quickly penetrate the insect body wall and efficiently silence gene expression. The formulation may provide a fast and easy tool for gene silence in some tough insects and for pest control in the field.
Authors: Fabiano Touzdjian Pinheiro Kohlrausch Távora; Francisco de Assis Dos Santos Diniz; Camila de Moraes Rêgo-Machado; Natália Chagas Freitas; Fabrício Barbosa Monteiro Arraes; Eduardo Chumbinho de Andrade; Leila Lourenço Furtado; Karen Ofuji Osiro; Natália Lima de Sousa; Thiago Bérgamo Cardoso; Liliane Márcia Mertz Henning; Patrícia Abrão de Oliveira Molinari; Sérgio Enrique Feingold; Wayne B Hunter; Maria Fátima Grossi de Sá; Adilson Kenji Kobayashi; Alexandre Lima Nepomuceno; Thaís Ribeiro Santiago; Hugo Bruno Correa Molinari Journal: Front Bioeng Biotechnol Date: 2022-06-28