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Literature DB >> 30610723

Methods to Determine the Role of Autophagy Proteins in C. elegans Aging.

Sivan Henis-Korenblit¹, Alicia Meléndez^2,3.

Abstract

This chapter describes methods for the analysis of autophagy proteins in C. elegans aging. We discuss the strains to be considered, the methods for the delivery of double-stranded RNA, and the methods to measure autophagy levels, autophagic flux, and degradation by autophagy.

Entities: CellLine Chemical Disease Gene Mutation Species

Keywords: Aging; Autophagy; Caenorhabditis elegans; Double-stranded RNA; GFP::LGG-1; HT115; L4; Longevity; RNA interference; SQST-1::GFP; Transgene; dsRNA

Mesh：

Substances：

Year: 2019 PMID： 30610723 PMCID： PMC8210635 DOI： 10.1007/978-1-4939-8873-0_37

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Methods to Determine the Role of Autophagy Proteins in C. elegans Aging.

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