| Literature DB >> 30610698 |
Sigurdur Gudmundsson1, Jenny Kahlhofer2, Nastassia Baylac1, Katri Kallio1, Eeva-Liisa Eskelinen3.
Abstract
Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.Keywords: Autophagosome; Electron tomography; Live-cell imaging; Phagophore; Serial sectioning
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Year: 2019 PMID: 30610698 DOI: 10.1007/978-1-4939-8873-0_12
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745