| Literature DB >> 30610645 |
Zenpei Shimatani1, Tohru Ariizumi2, Ushio Fujikura1, Akihiko Kondo1,3, Hiroshi Ezura2, Keiji Nishida4.
Abstract
The Target-AID system, consisting of a complex of cytidine deaminase and deficient CRISPR/Cas9, enables highly specific genomic nucleotide substitutions without the need for template DNA. The Cas9-fused cytidine deaminase is guided by sgRNAs and catalyzes the conversion of cytosine to uracil. The resulting U-G DNA mismatches trigger nucleotide substitutions (C to T or G to A) through DNA replication and repair pathways. Target-AID also retains the benefits of conventional CRISPR/Cas9 including robustness in various organisms, high targeting efficiency, and multiplex simultaneous gene editing. Our research group recently developed plant-optimized Target-AID system and demonstrated targeted base editing in tomato and rice. In this chapter, we introduce methods for Target-AID application in tomato.Entities:
Keywords: Activation-induced cytidine deaminase (AID); CRISPR/Cas9; Target-AID; Targeted nucleotide substitution; Tomato
Mesh：
Substances：
Year: 2019 PMID： 30610645 DOI： 10.1007/978-1-4939-8991-1_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745