| Literature DB >> 30610641 |
Yingxiao Zhang1, Yong Zhang2, Yiping Qi3,4.
Abstract
CRISPR-Cpf1 (Cas12a) is a class II type V endonuclease, which has been used as a genome editing tool in different biological systems. Here we describe a fast, efficient, and user-friendly system for CRISPR-Cpf1 expression vector assembly. In this system, the Pol II promoter is used to drive the expression of both Cpf1 and its crRNA, with the crRNA flanked by hammerhead (HH) and hepatitis delta virus (HDV) ribozyme RNAs for precise crRNA processing. All the components of this system can be modified depending on plant species and experimental goals. Using this system, nearly 100% editing efficiency and 90% gene expression decrease were achieved in rice and Arabidopsis, respectively.Entities:
Keywords: CRISPR-Cpf1 (Cas12a); Gateway cloning; Plant gene knockdown; Plant gene knockout
Mesh：
Year: 2019 PMID： 30610641 DOI： 10.1007/978-1-4939-8991-1_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745