| Literature DB >> 30610640 |
Hongge Jia1, Xiuping Zou1, Vladimir Orbovic2, Nian Wang3.
Abstract
CRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be created by Agrobacterium-mediated epicotyl transformation.Keywords: Agrobacterium-mediated epicotyl transformation; Cas9/sgRNA; Citrus; CsLOB1; Genome editing
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Year: 2019 PMID： 30610640 DOI： 10.1007/978-1-4939-8991-1_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745