Cristina Stasi1, Emmanuel A Tsochatzis2, Andrew Hall3, William Rosenberg4, Stefano Milani5, Amar Paul Dhillon3, Massimo Pinzani2. 1. Department of Experimental and Clinical Medicine, Careggi University Hospital, Florence, Italy. Electronic address: cristina.stasi@gmail.com. 2. Regenerative Medicine and Fibrosis Group, Institute for Liver and Digestive Health, University College London, Royal Free Campus London, United Kingdom; Sheila Sherlock Liver Centre, Royal Free Hospital, London, United Kingdom. 3. Department of Cellular Pathology, UCL Medical School, Royal Free Campus, United Kingdom. 4. Institute for Liver and Digestive Health, Division of Medicine, University College London, London, United Kingdom. 5. Department of Biomedical, Experimental and Clinical Sciences "Mario Serio", Careggi University Hospital, Florence, Italy.
Abstract
BACKGROUND: Fibrosis progression is the common consequence of most chronic liver diseases. AIMS: To evaluate the performance of Collagen Proportionate Area (CPA) and ELF using Ishak's score in patients with chronic liver diseases. METHODS: Retrospective analysis of medical data from patients on whom a liver biopsy was performed as part of the diagnostic assessment. CPA was calculated by using digital image analysis and then compared with Ishak and ELF scores. RESULTS: 143 patients (84 men (59%); mean age 48.8 ± 12.8 years) were evaluated. Patients were mainly affected by viral hepatitis (92 HCV and 8 HBV). CPA and ELF values increased with worsening Ishak stage (P < 0.001) and their median values were significantly different among Ishak stages (P < 0.001). There was a significant correlation between CPA and ELF (r = 0.5). In AUROC analysis, CPA and ELF had similar diagnostic accuracy in identifying cirrhosis, but CPA had higher diagnostic accuracy than ELF in identifying significant or absent fibrosis. High ELF scores were observed in non-cirrhotic patients who suffered non-liver related deaths. CONCLUSIONS: This study demonstrated that CPA and ELF values successfully identified patients with advanced fibrosis or cirrhosis, thus confirming the role of ELF as a clinical method for non-invasive assessment of fibrosis stage in chronic hepatitis.
BACKGROUND:Fibrosis progression is the common consequence of most chronic liver diseases. AIMS: To evaluate the performance of Collagen Proportionate Area (CPA) and ELF using Ishak's score in patients with chronic liver diseases. METHODS: Retrospective analysis of medical data from patients on whom a liver biopsy was performed as part of the diagnostic assessment. CPA was calculated by using digital image analysis and then compared with Ishak and ELF scores. RESULTS: 143 patients (84 men (59%); mean age 48.8 ± 12.8 years) were evaluated. Patients were mainly affected by viral hepatitis (92 HCV and 8 HBV). CPA and ELF values increased with worsening Ishak stage (P < 0.001) and their median values were significantly different among Ishak stages (P < 0.001). There was a significant correlation between CPA and ELF (r = 0.5). In AUROC analysis, CPA and ELF had similar diagnostic accuracy in identifying cirrhosis, but CPA had higher diagnostic accuracy than ELF in identifying significant or absent fibrosis. High ELF scores were observed in non-cirrhotic patients who suffered non-liver related deaths. CONCLUSIONS: This study demonstrated that CPA and ELF values successfully identified patients with advanced fibrosis or cirrhosis, thus confirming the role of ELF as a clinical method for non-invasive assessment of fibrosis stage in chronic hepatitis.
Authors: David Marti-Aguado; Matías Fernández-Patón; Clara Alfaro-Cervello; Claudia Mestre-Alagarda; Mónica Bauza; Ana Gallen-Peris; Víctor Merino; Salvador Benlloch; Judith Pérez-Rojas; Antonio Ferrández; Víctor Puglia; Marta Gimeno-Torres; Victoria Aguilera; Cristina Monton; Desamparados Escudero-García; Ángel Alberich-Bayarri; Miguel A Serra; Luis Marti-Bonmati Journal: Biomolecules Date: 2021-12-02