K Xu1, J L Wang2, M P Chu3, C Jia4. 1. The Institute of Life Sciences, Wenzhou University, Wenzhou, Zhejiang, China. Electronic address: godxu1987@163.com. 2. Department of Clinical Laboratory, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address: 407434006@qq.com. 3. Pediatric Research Institute, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; Children's Heart Center, Institute of Cardiovascular Development and Translational Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address: chmping@hotmail.com. 4. Pediatric Research Institute, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address: jiashang0802@163.com.
Abstract
OBJECTIVE: The aim of this study was to investigate the antibiofilm activity of coumarin against Candida albicans. METHODS: The efficacy of coumarin against biofilm formation and the mature biofilm of C. albicans was quantified by crystal violet (CV) staining and the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay. The effect of coumarin on C. albicans adhesion was assessed on polystyrene plates and by using the cell surface hydrophobicity (CSH) assay. The morphological transition of C. albicans was conducted in two types of hyphae-inducing media at 37°C. The expression of hypha/biofilm-related genes was evaluated using qRT-PCR analysis. A rescue experiment involving addition of exogenous cyclic adenosine monophosphate (cAMP) was performed to investigate the involvement of cAMP in the yeast-to-hyphae transition. A C. albicans-infected Caenorhabditis elegans model was used to test the anti-virulence efficacy of coumarin. RESULTS: Treatment with coumarin strongly affected the capacity of C. albicans to form biofilm and significantly impaired the preformed mature biofilm. The addition of coumarin notably inhibited C. albicans adhesion, CSH, and filamentation. The expression of some adhesion- and hypha-related genes, including HWP1, HYR1, ECE1, and ALS3, was remarkably down-regulated upon exposure to coumarin. Supplementation with cAMP partly rescued the coumarin-induced defects in hyphal development. Finally, coumarin prolonged survival in C. albicans-infected nematodes. CONCLUSION: Coumarin inhibited C. albicans biofilm, which was associated with attenuated adhesion and hyphal growth.
OBJECTIVE: The aim of this study was to investigate the antibiofilm activity of coumarin against Candida albicans. METHODS: The efficacy of coumarin against biofilm formation and the mature biofilm of C. albicans was quantified by crystal violet (CV) staining and the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay. The effect of coumarin on C. albicans adhesion was assessed on polystyrene plates and by using the cell surface hydrophobicity (CSH) assay. The morphological transition of C. albicans was conducted in two types of hyphae-inducing media at 37°C. The expression of hypha/biofilm-related genes was evaluated using qRT-PCR analysis. A rescue experiment involving addition of exogenous cyclic adenosine monophosphate (cAMP) was performed to investigate the involvement of cAMP in the yeast-to-hyphae transition. A C. albicans-infected Caenorhabditis elegans model was used to test the anti-virulence efficacy of coumarin. RESULTS: Treatment with coumarin strongly affected the capacity of C. albicans to form biofilm and significantly impaired the preformed mature biofilm. The addition of coumarin notably inhibited C. albicans adhesion, CSH, and filamentation. The expression of some adhesion- and hypha-related genes, including HWP1, HYR1, ECE1, and ALS3, was remarkably down-regulated upon exposure to coumarin. Supplementation with cAMP partly rescued the coumarin-induced defects in hyphal development. Finally, coumarin prolonged survival in C. albicans-infected nematodes. CONCLUSION:Coumarin inhibited C. albicans biofilm, which was associated with attenuated adhesion and hyphal growth.
Authors: Jhones do Nascimento Dias; Calliandra de Souza Silva; Alyne Rodrigues de Araújo; Jessica Maria Teles Souza; Paulo Henrique de Holanda Veloso Júnior; Wanessa Felix Cabral; Maria da Glória da Silva; Peter Eaton; José Roberto de Souza de Almeida Leite; André Moraes Nicola; Patrícia Albuquerque; Ildinete Silva-Pereira Journal: Sci Rep Date: 2020-06-25 Impact factor: 4.379
Authors: María Coronada Fernández-Calderón; Laura Hernández-González; Carolina Gómez-Navia; María Teresa Blanco-Blanco; Rosa Sánchez-Silos; Leopoldo Lucio; Ciro Pérez-Giraldo Journal: BMC Complement Med Ther Date: 2021-05-21
Authors: Sara Thamires Dias da Fonseca; Thaiz Rodrigues Teixeira; Jaqueline Maria Siqueira Ferreira; Luciana Alves Rodrigues Dos Santos Lima; Walter Luyten; Ana Hortência Fonsêca Castro Journal: Plants (Basel) Date: 2022-07-07