Literature DB >> 3060469

Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones.

J P Bennett1, R A Cross, J Kendrick-Jones, A G Weeds.   

Abstract

We have purified a polyclonal antibody by affinity chromatography which binds specifically to the phosphorylated form of the regulatory light chain (Mr = 20,000) of smooth muscle myosin. This antibody does not stain relaxed, permeabilized smooth muscle cells isolated from guinea pig taenia coli. However, when these cells were stimulated to contract with CaCl2 (100 microM) and ATP (1 mM), the immunofluorescence staining was localized in a series of transverse bands. This distribution of activated myosin appears to reflect an underlying structural organization of the smooth muscle cell cytoskeleton into mechanically coupled contractile zones.

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Year:  1988        PMID： 3060469      PMCID： PMC2115625          DOI： 10.1083/jcb.107.6.2623

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  31 in total

1.  Phosphorylation of smooth muscle myosin light chain kinase by the catalytic subunit of adenosine 3': 5'-monophosphate-dependent protein kinase.

Authors:  R S Adelstein; M A Conti; D R Hathaway; C B Klee
Journal:  J Biol Chem       Date:  1978-12-10       Impact factor: 5.157

2.  What is 10S myosin for?

Authors:  R A Cross
Journal:  J Muscle Res Cell Motil       Date:  1988-02       Impact factor: 2.698

3.  Analysis of the birefringence of the smooth muscle anococcygeus of the rat, at rest and in contraction. I.

Authors:  A Godfraind-De Becker; J M Gillis
Journal:  J Muscle Res Cell Motil       Date:  1988-02       Impact factor: 2.698

4.  Contractile units in vertebrate smooth muscle cells.

Authors:  J V Small
Journal:  Nature       Date:  1974-05-24       Impact factor: 49.962

5.  Cytoplasmic microtubular images in glutaraldehyde-fixed tissue culture cells by electron microscopy and by immunofluorescence microscopy.

Authors:  K Weber; P C Rathke; M Osborn
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

6.  An electrophoretic study of the low-molecular-weight components of myosin.

Authors:  W T Perrie; S V Perry
Journal:  Biochem J       Date:  1970-08       Impact factor: 3.857

7.  SDS microslab linear gradient polyacrylamide gel electrophoresis.

Authors:  P T Matsudaira; D R Burgess
Journal:  Anal Biochem       Date:  1978-07-01       Impact factor: 3.365

8.  Production and characterization of an antibody to myosin light chain kinase and intracellular localization of the enzyme.

Authors:  V Guerriero; D R Rowley; A R Means
Journal:  Cell       Date:  1981-12       Impact factor: 41.582

9.  Density of myosin filaments in the rat anococcygeus muscle, at rest and in contraction. II.

Authors:  J M Gillis; M L Cao; A Godfraind-De Becker
Journal:  J Muscle Res Cell Motil       Date:  1988-02       Impact factor: 2.698

10.  An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy.

Authors:  J G White; W B Amos; M Fordham
Journal:  J Cell Biol       Date:  1987-07       Impact factor: 10.539
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  5 in total

1.  Regulation of myosin II dynamics by phosphorylation and dephosphorylation of its light chain in epithelial cells.

Authors:  Toshiyuki Watanabe; Hiroshi Hosoya; Shigenobu Yonemura
Journal:  Mol Biol Cell       Date:  2006-12-06       Impact factor: 4.138

2.  Antibodies probe for folded monomeric myosin in relaxed and contracted smooth muscle.

Authors:  A Horowitz; K M Trybus; D S Bowman; F S Fay
Journal:  J Cell Biol       Date:  1994-09       Impact factor: 10.539

3.  The cytoskeletal and contractile apparatus of smooth muscle: contraction bands and segmentation of the contractile elements.

Authors:  A Draeger; W B Amos; M Ikebe; J V Small
Journal:  J Cell Biol       Date:  1990-12       Impact factor: 10.539

4.  Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells.

Authors:  F Matsumura; S Ono; Y Yamakita; G Totsukawa; S Yamashiro
Journal:  J Cell Biol       Date:  1998-01-12       Impact factor: 10.539

Review 5.  Regulation of intraocular pressure by soluble and membrane guanylate cyclases and their role in glaucoma.

Authors:  Emmanuel S Buys; Lincoln R Potter; Louis R Pasquale; Bruce R Ksander
Journal:  Front Mol Neurosci       Date:  2014-05-19       Impact factor: 5.639
  5 in total

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