BACKGROUND: Female reproductive tract microbiota may affect human reproduction. The current study considered whether a more detailed characterization of the vaginal microbiota could improve prediction of risk of poor reproductive outcome in patients undergoing in vitro fertilization (IVF). METHODS: Vaginal samples from 120 patients undergoing IVF were sequenced using the V4 region of the 16S ribosomal RNA gene with clustering of Gardnerella vaginalis genomic clades. Abnormal vaginal microbiota was defined by microscopy and quantitative polymerase chain reaction (qPCR) for G. vaginalis and/or Atopobium vaginae above a threshold. RESULTS: Three major community state types with abundance of Lactobacillus crispatus, Lactobacillus iners, and a diverse community type were identified, including 2 subtypes, characterized by a high abundance of L. crispatus and L. iners, respectively, but in combination with common diversity type operational taxonomic units. No significant association between community state type and the reproductive outcome could be demonstrated; however, abnormal vaginal microbiota by qPCR and a grouping based on high Shannon diversity index predicted the reproductive outcome equally well. CONCLUSIONS: The predictive value of 16S ribosomal RNA gene sequencing was not superior to the simpler and less expensive qPCR diagnostic approach in predicting the risk of a poor reproductive outcome in patients undergoing IVF. CLINICAL TRIALS REGISTRATION: NCT02042352.
BACKGROUND: Female reproductive tract microbiota may affect human reproduction. The current study considered whether a more detailed characterization of the vaginal microbiota could improve prediction of risk of poor reproductive outcome in patients undergoing in vitro fertilization (IVF). METHODS: Vaginal samples from 120 patients undergoing IVF were sequenced using the V4 region of the 16S ribosomal RNA gene with clustering of Gardnerella vaginalis genomic clades. Abnormal vaginal microbiota was defined by microscopy and quantitative polymerase chain reaction (qPCR) for G. vaginalis and/or Atopobium vaginae above a threshold. RESULTS: Three major community state types with abundance of Lactobacillus crispatus, Lactobacillus iners, and a diverse community type were identified, including 2 subtypes, characterized by a high abundance of L. crispatus and L. iners, respectively, but in combination with common diversity type operational taxonomic units. No significant association between community state type and the reproductive outcome could be demonstrated; however, abnormal vaginal microbiota by qPCR and a grouping based on high Shannon diversity index predicted the reproductive outcome equally well. CONCLUSIONS: The predictive value of 16S ribosomal RNA gene sequencing was not superior to the simpler and less expensive qPCR diagnostic approach in predicting the risk of a poor reproductive outcome in patients undergoing IVF. CLINICAL TRIALS REGISTRATION: NCT02042352.
Authors: A M Eskew; M J Stout; B S Bedrick; J K Riley; K R Omurtag; P T Jimenez; R R Odem; V S Ratts; S L Keller; E S Jungheim; K M Wylie Journal: BJOG Date: 2019-10-20 Impact factor: 6.531
Authors: Erica M Lokken; Lisa E Manhart; John Kinuthia; James P Hughes; Clayton Jisuvei; Khamis Mwinyikai; Charles H Muller; Kishor Mandaliya; Walter Jaoko; R Scott McClelland Journal: Hum Reprod Date: 2021-04-20 Impact factor: 6.918
Authors: Erica M Lokken; Clayton Jisuvei; James P Hughes; Kishor Mandaliya; Lisa E Manhart; Khamis Mwinyikai; Charles H Muller; Walter Jaoko; John Kinuthia; R Scott McClelland Journal: Fertil Steril Date: 2022-01-17 Impact factor: 7.329
Authors: Madeline E Graham; William G Herbert; Stephanie D Song; Harshini N Raman; Jade E Zhu; Paulina E Gonzalez; Marina R S Walther-António; Marc J Tetel Journal: Trends Endocrinol Metab Date: 2021-05-25 Impact factor: 10.586