Shi Liming1, Li Guixia1, Shi Wenxin2, Tian Guirong2. 1. a Department of Clinical Laboratory , Heze municipal Hospital , Heze , China. 2. b Department of Scientific Research and Teaching , Heze municipal Hospital , Heze , China.
Abstract
PURPOSE OF THE STUDY: Pulmonary infection with Klebsiella pneumoniae (KP) and carbapenem-resistant Klebsiella pneumoniae (CRKP) significantly contribute to morbidity and mortality in pneumonia. Recent studies have indicated that High-Mobility Group Box 1 Protein (HMGB1) plays an important role in the prevention and treatment of pneumonia. However the role of HMGB1 in CRKP-induced pneumonia has not been addressed. Materials and Methods: In vivo, we successfully established the KP and CRKP-induced pneumonia mouse model. We then tested the anti-HMGB1 IgG prevents CRKP-induced pneumonia. RESULTS: The mice treated with the anti-HMGB1 IgG ameliorated CRKP-induced pulmonary infiltration of inflammatory cells, dissemination of bacteria and the cytokine storm by suppressing the HMGB1 signaling pathways. CONCLUSION: These results indicate that HMGB1 may be an important contributor in these changes of CRKP-induced pneumonia. Thus, HMGB1 may provide a therapeutic target for reducing bacterial infection and lung inflammation in CRKP pneumonia.
PURPOSE OF THE STUDY: Pulmonary infection with Klebsiella pneumoniae (KP) and carbapenem-resistant Klebsiella pneumoniae (CRKP) significantly contribute to morbidity and mortality in pneumonia. Recent studies have indicated that High-Mobility Group Box 1 Protein (HMGB1) plays an important role in the prevention and treatment of pneumonia. However the role of HMGB1 in CRKP-induced pneumonia has not been addressed. Materials and Methods: In vivo, we successfully established the KP and CRKP-induced pneumoniamouse model. We then tested the anti-HMGB1 IgG prevents CRKP-induced pneumonia. RESULTS: The mice treated with the anti-HMGB1 IgG ameliorated CRKP-induced pulmonary infiltration of inflammatory cells, dissemination of bacteria and the cytokine storm by suppressing the HMGB1 signaling pathways. CONCLUSION: These results indicate that HMGB1 may be an important contributor in these changes of CRKP-induced pneumonia. Thus, HMGB1 may provide a therapeutic target for reducing bacterial infection and lung inflammation in CRKP pneumonia.
Authors: Mao Wang; Alex G Gauthier; Thomas P Kennedy; Haichao Wang; Uday Kiran Velagapudi; Tanaji T Talele; Mosi Lin; Jiaqi Wu; LeeAnne Daley; Xiaojing Yang; Vivek Patel; Sung Soo Mun; Charles R Ashby; Lin L Mantell Journal: Mol Med Date: 2021-07-16 Impact factor: 6.354