Preparation of chitosan-based nanoparticles for enzyme immobilization.

The aim of the present work was to prepare high loading capacity carriers for immobilizing glucoamylase. Different sizes of chitosan based particles were successfully prepared by different methods to evaluate the performance in immobilization. Chitosan particles on millimeter size were prepared by dripping granulation method, chitosan covered magnetic nanoparticles and chitosan mixted graphene oxide nanosheets covered magnetic nanoparticles were synthesized by one-step method, chitosan-glucoamylase nanoparticles were synthesized by ionic cross linking method with Sodium tripolyphosphate. These particles were characterized by SEM, TEM, FTIR and DLS analysis. The performance of the immobilized enzyme was also investigated. The results showed that the loading capacity was greatly increased on chitosan based nanoparticles. The reaction conditions of immobilized enzyme were optimized, the reusability and storage stability was also investigated. The results showed the pH durance and storage stability of the immobilized enzyme on nanosize particles were enhanced.