Denis Leméteil1,2, Gilles Gargala1,2, Romy Razakandrainibe2, Jean Jacques Ballet2, Loic Favennec1,2, Damien Costa1,2.
Abstract
BACKGROUND: Effective stool concentration is essential in microscopically based diagnosis of human intestinal parasite infections.
OBJECTIVE: To compare the performances of 4 concentration commercial kits and 1 homemade procedure in 96 clinical stool specimens that tested positive for the detection of 9 helminth and 8 protozoa parasites.
METHODS: The presence or absence of parasite forms was microscopically determined under conditions of standard practice. Also, we established the accuracies, concentration factors, and extraction yields.
RESULTS: No difference was observed between procedures for preconcentration specimens that tested positive. However, for preconcentration specimens that tested negative, we discovered that the homemade procedure was the most effective, and 2 of the 4 commercial kits were discovered to be satisfactory for routine applications.
CONCLUSIONS: For all parasites, procedures with biphasic solvents exhibited higher performances than organic solvent-free procedures. For the first time, the effectiveness of commercial concentration kits has been evaluated on several common stool parasites, and the results suggest that improvement of commercial procedures is possible. © American Society for Clinical Pathology 2018. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
BACKGROUND: Effective stool concentration is essential in microscopically based diagnosis of human intestinal parasite infections.
OBJECTIVE: To compare the performances of 4 concentration commercial kits and 1 homemade procedure in 96 clinical stool specimens that tested positive for the detection of 9 helminth and 8 protozoa parasites.
METHODS: The presence or absence of parasite forms was microscopically determined under conditions of standard practice. Also, we established the accuracies, concentration factors, and extraction yields.
RESULTS: No difference was observed between procedures for preconcentration specimens that tested positive. However, for preconcentration specimens that tested negative, we discovered that the homemade procedure was the most effective, and 2 of the 4 commercial kits were discovered to be satisfactory for routine applications.
CONCLUSIONS: For all parasites, procedures with biphasic solvents exhibited higher performances than organic solvent-free procedures. For the first time, the effectiveness of commercial concentration kits has been evaluated on several common stool parasites, and the results suggest that improvement of commercial procedures is possible. © American Society for Clinical Pathology 2018. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Entities:
Keywords:
concentration; detection; helminth; parasite; protozoa; stool
Year: 2019
PMID： 30590699 DOI： 10.1093/labmed/lmy072
Source DB: PubMed Journal: Lab Med ISSN： 0007-5027