Patricia Rivera1, María T Ramírez-López2,3, Antonio Vargas4, Juan Decara4, Mariam Vázquez4, Rocío Arco4, Raquel Gómez de Heras2, Jesús Argente1,5,6,7, Fernando Rodríguez de Fonseca2,4, Julie A Chowen1,5,6, Juan Suárez4. 1. Department of Endocrinology, Fundación Investigación Biomédica del Hospital Infantil Universitario Niño Jesús, Instituto de Investigación Biomédica la Princesa, Madrid, Spain. 2. Departamento de Psicobiología, Facultad de Psicología, Universidad Complutense de Madrid, Pozuelo de Alarcón, Spain. 3. Hospital Universitario de Getafe, Getafe (Madrid), Spain. 4. Unidad de Gestión Clínica de Salud Mental, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. 5. Centro de Investigación Biomédica en Red Fisiología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain. 6. IMDEA Food Institute, Campus of International Excellence (CEI) UAM + CSIC, Madrid, Spain. 7. Department of Pediatrics, University Autonoma de Madrid, Madrid, Spain.
Abstract
AIM: We aimed to investigate whether a dysregulated maternal diet during gestation and lactation induces long-lasting changes in the hypothalamic control of feeding behavior in the offspring and whether this effect is sex specific. METHODS: The study included an analysis of appetite-regulating metabolic hormones and hypothalamic signaling in male and female offspring in adulthood after exposure to a free-choice high-calorie palatable low-protein (P) diet or standard chow (C) during (pre)gestation/lactation (maternal) and/or postweaning (offspring). RESULTS: Maternal exposure to the P diet resulted in decreased protein intake and body weight gain in dams and decreased body weight gain in offspring during lactation. The maternal P diet (PC) specifically increased feed efficacy and decreased body weight and cholesterol levels in the female offspring in adulthood, but no changes in adiposity or leptin levels were found. In contrast, P diet exposure after weaning (CP and PP) increased caloric intake, adiposity and circulating levels of leptin in the male and female offspring in adulthood. The hypothalami of the female offspring exposed to the maternal P diet (PC and PP) expressed high levels of the phospho-leptin receptor and low levels of SOCS3, phospho-IRS1 and phospho-AMPK, regardless of the postweaning diet. The hypothalami of the female rats in the PC group also showed increased levels of STAT3 and the orexigenic neuropeptide Agrp. CONCLUSIONS: Maternal exposure to a free-choice high-calorie low-protein diet induces a long-term feed efficacy associated with changes in leptin signaling through IRS-1 and AMPK dephosphorylation in the hypothalami of female offspring in adulthood.
AIM: We aimed to investigate whether a dysregulated maternal diet during gestation and lactation induces long-lasting changes in the hypothalamic control of feeding behavior in the offspring and whether this effect is sex specific. METHODS: The study included an analysis of appetite-regulating metabolic hormones and hypothalamic signaling in male and female offspring in adulthood after exposure to a free-choice high-calorie palatable low-protein (P) diet or standard chow (C) during (pre)gestation/lactation (maternal) and/or postweaning (offspring). RESULTS: Maternal exposure to the P diet resulted in decreased protein intake and body weight gain in dams and decreased body weight gain in offspring during lactation. The maternal P diet (PC) specifically increased feed efficacy and decreased body weight and cholesterol levels in the female offspring in adulthood, but no changes in adiposity or leptin levels were found. In contrast, P diet exposure after weaning (CP and PP) increased caloric intake, adiposity and circulating levels of leptin in the male and female offspring in adulthood. The hypothalami of the female offspring exposed to the maternal P diet (PC and PP) expressed high levels of the phospho-leptin receptor and low levels of SOCS3, phospho-IRS1 and phospho-AMPK, regardless of the postweaning diet. The hypothalami of the female rats in the PC group also showed increased levels of STAT3 and the orexigenic neuropeptide Agrp. CONCLUSIONS: Maternal exposure to a free-choice high-calorie low-protein diet induces a long-term feed efficacy associated with changes in leptin signaling through IRS-1 and AMPK dephosphorylation in the hypothalami of female offspring in adulthood.
Authors: Noemí Blanco; Jose Manuel Fernández-García; Beatriz Carrillo; Antonio Ballesta; Rocío García-Úbeda; Paloma Collado; Helena Pinos Journal: Front Neuroanat Date: 2022-06-16 Impact factor: 3.543
Authors: Patricia Rivera; Rubén Tovar; María Teresa Ramírez-López; Juan Antonio Navarro; Antonio Vargas; Juan Suárez; Fernando Rodríguez de Fonseca Journal: Nutrients Date: 2020-06-19 Impact factor: 5.717
Authors: Antonio J López-Gambero; Cristina Rosell-Valle; Dina Medina-Vera; Juan Antonio Navarro; Antonio Vargas; Patricia Rivera; Carlos Sanjuan; Fernando Rodríguez de Fonseca; Juan Suárez Journal: Int J Mol Sci Date: 2021-05-20 Impact factor: 5.923
Authors: Rubén Tovar; Antonio Vargas; Jesús Aranda; Lourdes Sánchez-Salido; Laura González-González; Julie A Chowen; Fernando Rodríguez de Fonseca; Juan Suárez; Patricia Rivera Journal: Int J Mol Sci Date: 2021-06-11 Impact factor: 5.923