Kuang-Chi Lai1,2, Shu-Fen Peng3, Chia-Chi Liu4,5, Jye-Yu Huang6, Jung-Yu Kuo6, Zheng-Yu Cheng6, Rick Sai-Chuen Wu7,8, Chin-Chung Lin9,10, Jr-Kai Chen11, Jing-Gung Chung12,13. 1. Department of Medical Laboratory Science and Biotechnology, College of Medicine and Life Science, Chung Hwa University of Medical Technology, Tainan, Taiwan, R.O.C. 2. Department of Surgery, China Medical University Beigang Hospital, Beigang, Taiwan, R.O.C. 3. Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C. 4. Department of Biochemical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan, R.O.C. 5. Cardiovascular Center, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C. 6. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. 7. Department of Anesthesiology, China Medical University Hospital, Taichung, Taiwan, R.O.C. 8. Department of Anesthesiology, China Medical University, Taichung, Taiwan, R.O.C. 9. Department of Chinese Medicine, Feng-Yuan Hospital, Ministry of Health and Welfare, Executive Yuan, Taichung, Taiwan, R.O.C. 10. General Education Center, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. 11. Department of Orthopaedics, Chang Bing Show-Chwan Memorial Hospital, Changhua, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw u9101046@hotmail.com. 12. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw u9101046@hotmail.com. 13. Department of Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: Maslinic acid (MA), a pentacyclic triterpene extracted from wax-like coatings of olives, has been shown to reduce cancer cell number through induction of autophagy and apoptosis in many human cancer cells including human leukemia HL-60 cells. In the present study, we investigated whether or not MA affects immune responses in a leukemia mouse model. MATERIALS AND METHODS: WEHI-3 cells were intraperitonealIy (i.p.) injected into normal BALB/c mice to develop leukemia. Mice were then treated by i.p. injection with MA at different doses (0, 8, 16 and 32 mg/kg) for 2 weeks. After treatment, all animals were weighed and blood, liver and spleen tissues were weighed. Blood or spleen both were used for determination of cell markers or phagocytosis, natural killer (NK) cell activities and T- and B-cell proliferation, respectively, by using a flow cytometric assay. RESULTS: MA did not significantly affect body, liver, and spleen weights. However, MA increased markers of T-cells (at 16 mg/kg treatment) and monocytes (at 32 mg/kg treatment), but reduced B-cell markers (at 8 mg/kg treatment); MA did not significantly affect cell marker of macrophages. Furthermore, MA increased phagocytosis by macrophages from peripheral blood mononuclear cells and peritoneal cavity at 32 mg/kg treatment and increased NK cell activity at target cell:splenocyte ratio of 25:1 but did not affect B- and T-cell proliferation. CONCLUSION: MA increased immune responses by enhancing macrophage phagocytosis and NK cell activities in leukemic mice. Copyright
BACKGROUND/AIM: Maslinic acid (MA), a pentacyclic triterpene extracted from wax-like coatings of olives, has been shown to reduce cancer cell number through induction of autophagy and apoptosis in many humancancer cells including humanleukemia HL-60 cells. In the present study, we investigated whether or not MA affects immune responses in a leukemiamouse model. MATERIALS AND METHODS: WEHI-3 cells were intraperitonealIy (i.p.) injected into normal BALB/c mice to develop leukemia. Mice were then treated by i.p. injection with MA at different doses (0, 8, 16 and 32 mg/kg) for 2 weeks. After treatment, all animals were weighed and blood, liver and spleen tissues were weighed. Blood or spleen both were used for determination of cell markers or phagocytosis, natural killer (NK) cell activities and T- and B-cell proliferation, respectively, by using a flow cytometric assay. RESULTS: MA did not significantly affect body, liver, and spleen weights. However, MA increased markers of T-cells (at 16 mg/kg treatment) and monocytes (at 32 mg/kg treatment), but reduced B-cell markers (at 8 mg/kg treatment); MA did not significantly affect cell marker of macrophages. Furthermore, MA increased phagocytosis by macrophages from peripheral blood mononuclear cells and peritoneal cavity at 32 mg/kg treatment and increased NK cell activity at target cell:splenocyte ratio of 25:1 but did not affect B- and T-cell proliferation. CONCLUSION: MA increased immune responses by enhancing macrophage phagocytosis and NK cell activities in leukemicmice. Copyright
Authors: Aixa Aguilera-Garrido; Elena Arranz; María José Gálvez-Ruiz; Juan Antonio Marchal; Francisco Galisteo-González; Linda Giblin Journal: Drug Deliv Date: 2022-12 Impact factor: 6.819