Ewa Bilska-Zając1, Mirosław Różycki2, Ewelina Antolak3, Aneta Bełcik4, Katarzyna Grądziel-Krukowska5, Jacek Karamon6, Jacek Sroka7, Jolanta Zdybel8, Tomasz Cencek9. 1. National Veterinary Research Institute, Puławy, Poland. biliwkazdejchwili@vp.pl. 2. National Veterinary Research Institute, Puławy, Poland. mrozycki@piwet.pulawy.pl. 3. National Veterinary Research Institute, Puławy, Poland. Ewelina.Antolak@piwet.pulawy. 4. National Veterinary Research Institute, Puławy, Poland. aneta.belcik@piwet.pulawy.pl. 5. National Veterinary Research Institute, Puławy, Poland. katarzyna.gradziel@piwet.pulawy.pl. 6. National Veterinary Research Institute, Puławy, Poland. jacek.karamon@piwet.pulawy.pl. 7. Institute of Rural Health, Lublin, Poland. jacek.sroka@piwet.pulawy.pl. 8. National Veterinary Research Institute, Puławy, Poland. j.zdybel@piwet.pulawy.pl. 9. National Veterinary Research Institute, Puławy, Poland. tcencek@piwet.pulawy.pl.
Abstract
INTRODUCTION: The highest risk of trichinellosis for human is considered in eating meat products containing live larvae, mostly from wild boars or pigs. Spreading of <i>Trichinella</i> spp. may occur in various ways, one of which is transmission by vectors. The rat is considered to be the most common vector for <i>Trichinella</i> parasite. The population of rats living on pig farms can play an important role in maintaining or spreading the parasite to other animals. OBJECTIVE: The aim of presented survey was to investigate the occurrence of <i>Trichinella</i> spp. in rats on farms with pigs infected with this parasite. MATERIAL AND METHODS: From pig farms selected for study, the muscles of collected rats were investigated by magnetic stirrer digestion method to assess occurrence of <i>Trichinella</i> in the rat population. Isolated <i>Trichinella</i> parasites were identified under stereomicroscope and multiplex PCR were performed for species identification. RESULTS: Rats infected with <i>Trichinella</i> spp. were discovered on three of five investigated pig farms. The mean extent of invasion in rats from the studied farms was 23.33%. The calculated medium intensity of invasion was 4.09 lpg (larvae per gram) (SD 5.41). All larvae of <i>Trichinella</i> discovered from rats were identified as <i>T.spiralis</i>. CONCLUSIONS: The results obtained indicate that in farms with a high prevalence of <i>Trichinella</i> invasion in pigs there are very likely to be found rats infected by this nematode. This suggests possibility to maintain the invasion in herd and spread into neighborhood farms.
INTRODUCTION: The highest risk of trichinellosis for human is considered in eating meat products containing live larvae, mostly from wild boars or pigs. Spreading of <i>Trichinella</i> spp. may occur in various ways, one of which is transmission by vectors. The rat is considered to be the most common vector for <i>Trichinella</i> parasite. The population of rats living on pig farms can play an important role in maintaining or spreading the parasite to other animals. OBJECTIVE: The aim of presented survey was to investigate the occurrence of <i>Trichinella</i> spp. in rats on farms with pigs infected with this parasite. MATERIAL AND METHODS: From pig farms selected for study, the muscles of collected rats were investigated by magnetic stirrer digestion method to assess occurrence of <i>Trichinella</i> in the rat population. Isolated <i>Trichinella</i> parasites were identified under stereomicroscope and multiplex PCR were performed for species identification. RESULTS:Rats infected with <i>Trichinella</i> spp. were discovered on three of five investigated pig farms. The mean extent of invasion in rats from the studied farms was 23.33%. The calculated medium intensity of invasion was 4.09 lpg (larvae per gram) (SD 5.41). All larvae of <i>Trichinella</i> discovered from rats were identified as <i>T.spiralis</i>. CONCLUSIONS: The results obtained indicate that in farms with a high prevalence of <i>Trichinella</i> invasion in pigs there are very likely to be found rats infected by this nematode. This suggests possibility to maintain the invasion in herd and spread into neighborhood farms.
Authors: Ewa Bilska-Zajac; Daniele Tonanzi; Edoardo Pozio; Miroslaw Rozycki; Tomasz Cencek; Peter C Thompson; Benjamin M Rosenthal; Giuseppe La Rosa Journal: Parasit Vectors Date: 2021-07-09 Impact factor: 3.876