Renata Galetti1, Leonardo Neves Andrade2, Alessandro M Varani3, Ana Lúcia Costa Darini2. 1. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil. Electronic address: regaletti@hotmail.com. 2. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil. 3. Faculdade de Ciencias Agrárias e Veterinárias, Universidade Estadual Paulista 'Julio de Mesquita Filho', Jaboticabal, Brazil.
Abstract
OBJECTIVES: The purpose of this study was to investigate the resistome of an SPM-1-producing Pseudomonas aeruginosa ST277 isolate (HC84) from Brazil. METHODS: Whole-genome sequencing of P. aeruginosa HC84 was performed using an Ion Proton™ System. De novo assembly was carried out using CLC Genomics Workbench 8.0, and gene prediction was performed using the Prokka pipeline. RESULTS AND CONCLUSION: Here we describe the resistome of SPM-1-producing P. aeruginosa ST277 (HC84) consisting of 13 different antimicrobial resistance genes [blaSPM-1, rmtD, aacA4, aadA7, blaOXA-56, blaOXA-396, blaPAO, aph(3')-IIb, aac(6')-Ib-cr, crpP, catB7, cmx and fosA). This particular chromosomal pack of resistance genes is strongly associated with clonal dissemination and suggests an important role in the persistence of this clone in Brazilian nosocomial infections. For that reason, could we already consider the 'chromosomal pack of acquired resistance genes' like an 'ST277 intrinsic resistome'? This is an example of chromosomal accumulation of acquired resistance genes as well as integrative and conjugative elements into a successful bacterial pathogen and calls attention to the evolution of other species driving to insertion and persistence of multiple acquired resistance genes in the bacterial chromosome.
OBJECTIVES: The purpose of this study was to investigate the resistome of an SPM-1-producing Pseudomonas aeruginosa ST277 isolate (HC84) from Brazil. METHODS: Whole-genome sequencing of P. aeruginosa HC84 was performed using an Ion Proton™ System. De novo assembly was carried out using CLC Genomics Workbench 8.0, and gene prediction was performed using the Prokka pipeline. RESULTS AND CONCLUSION: Here we describe the resistome of SPM-1-producing P. aeruginosa ST277 (HC84) consisting of 13 different antimicrobial resistance genes [blaSPM-1, rmtD, aacA4, aadA7, blaOXA-56, blaOXA-396, blaPAO, aph(3')-IIb, aac(6')-Ib-cr, crpP, catB7, cmx and fosA). This particular chromosomal pack of resistance genes is strongly associated with clonal dissemination and suggests an important role in the persistence of this clone in Brazilian nosocomial infections. For that reason, could we already consider the 'chromosomal pack of acquired resistance genes' like an 'ST277 intrinsic resistome'? This is an example of chromosomal accumulation of acquired resistance genes as well as integrative and conjugative elements into a successful bacterial pathogen and calls attention to the evolution of other species driving to insertion and persistence of multiple acquired resistance genes in the bacterial chromosome.
Authors: Mila M Almeida; Meyvianne T Freitas; Tania W Folescu; Monica C Firmida; Ana Paula D'A Carvalho-Assef; Elizabeth A Marques; Robson S Leão Journal: Curr Microbiol Date: 2021-01-06 Impact factor: 2.188
Authors: Catalina Díaz-Ríos; Marta Hernández; David Abad; Laura Álvarez-Montes; Athanasia Varsaki; David Iturbe; Jorge Calvo; Alain A Ocampo-Sosa Journal: Antibiotics (Basel) Date: 2021-04-23