Ari P Kirshenbaum1, Emily Chabot2, Nick Gibney2. 1. Saint Michael's College, Department of Psychology and Neuroscience Program, Colchester, VT, 05439, United States. Electronic address: akirshenbaum@smcvt.edu. 2. Saint Michael's College, Department of Psychology and Neuroscience Program, Colchester, VT, 05439, United States.
Abstract
BACKGROUND: The startle response, pre-pulse presentation of startle, and habituation in adult zebrafish (danio rerio) have not been formerly characterized using the same motion detection equipment within an integrated procedure. NEW METHOD: The methods presented in this manuscript describe the use of a video tracking software used previously in the detection of conditioned immobility in rodents, but adapted for the purposes of tracking zebrafish movement. RESULTS: The results from a series of investigations demonstrate an effective tracking and quantification of the startle response, as well as evidence that stimulus-experience history alters the startle response in adult zebrafish. COMPARISON WITH EXISTING METHODS: This method of tracking zebrafish allows for the quantification on movement of a single subject, and the delivery of the startle stimulus can be synchronized with the motion-detection software to obtain a high temporal resolution that is not provided by other means of motion-detection tracking. CONCLUSION: Objective techniques for evaluating these basic modifications of the startle response (pre-pulse and habituation) may be helpful in future behavioral analysis as this species is rapidly becoming among the most commonly utilized in preclinical pharmacological assessment.
BACKGROUND: The startle response, pre-pulse presentation of startle, and habituation in adult zebrafish (danio rerio) have not been formerly characterized using the same motion detection equipment within an integrated procedure. NEW METHOD: The methods presented in this manuscript describe the use of a video tracking software used previously in the detection of conditioned immobility in rodents, but adapted for the purposes of tracking zebrafish movement. RESULTS: The results from a series of investigations demonstrate an effective tracking and quantification of the startle response, as well as evidence that stimulus-experience history alters the startle response in adult zebrafish. COMPARISON WITH EXISTING METHODS: This method of tracking zebrafish allows for the quantification on movement of a single subject, and the delivery of the startle stimulus can be synchronized with the motion-detection software to obtain a high temporal resolution that is not provided by other means of motion-detection tracking. CONCLUSION: Objective techniques for evaluating these basic modifications of the startle response (pre-pulse and habituation) may be helpful in future behavioral analysis as this species is rapidly becoming among the most commonly utilized in preclinical pharmacological assessment.
Authors: Madeline M Pantoni; Gerald M Herrera; Kaitlin R Van Alstyne; Stephan G Anagnostaras Journal: Front Behav Neurosci Date: 2020-06-03 Impact factor: 3.558
Authors: G Jean Harry; Sandra McBride; Shannah K Witchey; Sakina Mhaouty-Kodja; Alain Trembleau; Matthew Bridge; Anna Bencsik Journal: Front Toxicol Date: 2022-02-25