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Literature DB >> 3058649

LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney.

Abstract

This work describes a method for the immunolocalization of laminin on 1 micron-thick tissue sections using a postembedding immunofluorescence technique. Embedding of unfixed or formaldehyde-fixed mouse renal cortex in either of the acrylic resins LR-White or LR-Gold permitted reliable postembedding immunofluorescence staining for laminin. LR-White was heat-cured at 50 degrees C whereas LR-Gold was polymerized at -25 degrees C. A stronger immunostaining for laminin was obtained from tissue embedded in polymerized LR-Gold compared with the staining from tissue embedded in LR-White. Prerequisites for adequate postembedding immunostaining are the partial dehydration of the tissue (maximum ethanol concentration, 70%) and pepsin treatment of the tissue sections prior to performing the immunostaining reactions.

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Year: 1988 PMID： 3058649 DOI： 10.1007/bf01002428

Source DB: PubMed Journal: Histochem J ISSN： 0018-2214

8 in total

1. Laminin--a glycoprotein from basement membranes.

Authors: R Timpl; H Rohde; P G Robey; S I Rennard; J M Foidart; G R Martin
Journal: J Biol Chem Date: 1979-10-10 Impact factor: 5.157

2. Light and electron microscopical postembedding lectin histochemistry for WGA-binding sites in the renal cortex of the mouse embedded in polyhydroxy aromatic resins LR-White and LR-Gold.

Authors: R Herken; M Fussek; M Thies
Journal: Histochemistry Date: 1988

3. Methodological dependence in the ultrastructural immunolocalization of laminin in tubular basement membranes of the mouse kidney.

Authors: R Herken; B Manshausen; M Fussek; G Bonatz
Journal: Histochemistry Date: 1987

3 in total

1. Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits.

Authors: Kristina D Micheva; Stephen J Smith
Journal: Neuron Date: 2007-07-05 Impact factor: 17.173

2. Postembedding immunogold histochemistry for the localization of laminin and its E4 and P1 fragments in mouse kidney embedded in LR-White and LR-Gold.

Authors: R Herken; N Miosge
Journal: Histochem J Date: 1991-06

3. Appearance of laminin, heparan sulphate proteoglycan and collagen type IV during initial stages of vascularisation of the neuroepithelium of the mouse embryo.

Authors: R Herken; W Götz; M Thies
Journal: J Anat Date: 1990-04 Impact factor: 2.610

3 in total

LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney.

1. Laminin--a glycoprotein from basement membranes.

2. Light and electron microscopical postembedding lectin histochemistry for WGA-binding sites in the renal cortex of the mouse embedded in polyhydroxy aromatic resins LR-White and LR-Gold.

3. Methodological dependence in the ultrastructural immunolocalization of laminin in tubular basement membranes of the mouse kidney.

4. Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues.

5. Immunocytochemical enhancement of basement membrane antigens by pepsin: applications in diagnostic pathology.

6. Immunocytochemical demonstration of serine: pyruvate amino-transferase in peroxisomes and mitochondria of rat kidney.

7. Post-embedding immunolabelling. Some effects of tissue preparation on the antigenicity of plant proteins.

8. A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope.

1. Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits.

2. Postembedding immunogold histochemistry for the localization of laminin and its E4 and P1 fragments in mouse kidney embedded in LR-White and LR-Gold.

3. Appearance of laminin, heparan sulphate proteoglycan and collagen type IV during initial stages of vascularisation of the neuroepithelium of the mouse embryo.