Qitong Zhang1,2, Lili Zhang2, Biao Geng2, Jianrong Feng1, Shuhua Zhu2. 1. Department of Horticulture, College of Agriculture, Shihezi University, Shihezi, PR China. 2. College of Chemistry and Material Science, Shandong Agriculture University, Taian, PR China.
Abstract
BACKGROUND: Cold conditions can accelerate the production of reactive oxygen species (ROS), and excessive ROS may attack biological macromolecules, disrupt related signal pathways, induce oxidative stress and influence plant metabolism. The cross-talk between nitric oxide (NO) and abscisic acid (ABA) and the inhibitions by NO or ABA on oxidative damage have been reported in fruits. However, there are few reports about the roles of NO-ABA interactions in chilling stress and antioxidant defense in fruits during cold storage. This study was conducted to investigate the roles of NO, ABA and interactions between NO and ABA in response to chilling stress on peach fruit (Prunus persica (L.) Batsch, cv. 'Xintaihong'). RESULTS: Treatments with 15 µmol L-1 NO, 100 µmol L-1 ABA and 15 µmol L-1 NO + 5 mmol L-1 sodium tungstate solution could reduce ROS content, alleviate lipid peroxidation and enhance antioxidant enzyme activities and antioxidant capacities. However, treatments with 5 µmol L-1 potassium 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO), 5 mmol L-1 sodium tungstate and 100 µmol L-1 ABA + 5 µmol L-1 c-PTIO differentially blocked these protective effects and significantly increased ROS content and lipid peroxidation of peaches under low-temperature conditions. CONCLUSIONS: Application of exogenous ABA could increase the resistance to cold-induced oxidative stress by enhancing the efficiency of enzymatic and non-enzymatic systems, which were partially mediated by NO.
BACKGROUND: Cold conditions can accelerate the production of reactive oxygen species (ROS), and excessive ROS may attack biological macromolecules, disrupt related signal pathways, induce oxidative stress and influence plant metabolism. The cross-talk between nitric oxide (NO) and abscisic acid (ABA) and the inhibitions by NO or ABA on oxidative damage have been reported in fruits. However, there are few reports about the roles of NO-ABA interactions in chilling stress and antioxidant defense in fruits during cold storage. This study was conducted to investigate the roles of NO, ABA and interactions between NO and ABA in response to chilling stress on peach fruit (Prunus persica (L.) Batsch, cv. 'Xintaihong'). RESULTS: Treatments with 15 µmol L-1 NO, 100 µmol L-1 ABA and 15 µmol L-1 NO + 5 mmol L-1 sodium tungstate solution could reduce ROS content, alleviate lipid peroxidation and enhance antioxidant enzyme activities and antioxidant capacities. However, treatments with 5 µmol L-1 potassium 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO), 5 mmol L-1 sodium tungstate and 100 µmol L-1 ABA + 5 µmol L-1 c-PTIO differentially blocked these protective effects and significantly increased ROS content and lipid peroxidation of peaches under low-temperature conditions. CONCLUSIONS: Application of exogenous ABA could increase the resistance to cold-induced oxidative stress by enhancing the efficiency of enzymatic and non-enzymatic systems, which were partially mediated by NO.
Authors: Pablo Fernández-Cancelo; Ariadna Iglesias-Sanchez; Salvador Torres-Montilla; Albert Ribas-Agustí; Neus Teixidó; Manuel Rodriguez-Concepcion; Jordi Giné-Bordonaba Journal: Front Plant Sci Date: 2022-08-01 Impact factor: 6.627