Xiaowen Chi1, Beichen Ding2, Lijuan Zhang3, Jiawen Zhang3, Jianmei Wang3, Wei Zhang3. 1. Pediatric, The First Affiliated Hospital of Harbin Medical University, Harbin, China. 2. Department of Urology, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China. 3. Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Abstract
OBJECTIVES: We herein aimed to explore whether growth arrest-specific 5 (GAS5) promotes M1 macrophage polarization in childhood pneumonia and to investigate the underlying mechanism. METHODS: Relative GAS5 and miR-455-5p expression and suppressor of cytokine signaling 3 (SOCS3) messenger RNA level were examined using quantitative reverse transcription polymerase chain reaction. Protein expression of SOCS3 and the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway-related proteins was detected using western blot analysis. Luciferase activity assay was performed to test whether miR-455-5p could bind to GAS5 or SOCS3. The macrophage phenotype was determined using flow cytometry analysis and enzyme-linked immunosorbent assay. RESULTS: The macrophage polarization toward the M2 phenotype was observed in peripheral blood from pneumonia children. Furthermore, GAS5 and SOCS3 expression were upregulated but miR-455-5p downregulated in human monocyte-derived macrophages from pneumonia children compared with the control group. Furthermore, GAS5 acted as a sponge for miR-455-5p to facilitate SOCS3 expression. Moreover, miR-455-5p mimic and SOCS3 knockdown significantly reversed the GAS5 overexpression-mediated suppression of the JAK2/STAT3 signaling and promotion of M1 polarization. CONCLUSION: GAS5 promotes M1 macrophage polarization by acting as a competing endogenous RNA of miR-455-5p to facilitate SOCS3 expression in childhood pneumonia.
OBJECTIVES: We herein aimed to explore whether growth arrest-specific 5 (GAS5) promotes M1 macrophage polarization in childhood pneumonia and to investigate the underlying mechanism. METHODS: Relative GAS5 and miR-455-5p expression and suppressor of cytokine signaling 3 (SOCS3) messenger RNA level were examined using quantitative reverse transcription polymerase chain reaction. Protein expression of SOCS3 and the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway-related proteins was detected using western blot analysis. Luciferase activity assay was performed to test whether miR-455-5p could bind to GAS5 or SOCS3. The macrophage phenotype was determined using flow cytometry analysis and enzyme-linked immunosorbent assay. RESULTS: The macrophage polarization toward the M2 phenotype was observed in peripheral blood from pneumoniachildren. Furthermore, GAS5 and SOCS3 expression were upregulated but miR-455-5p downregulated in human monocyte-derived macrophages from pneumoniachildren compared with the control group. Furthermore, GAS5 acted as a sponge for miR-455-5p to facilitate SOCS3 expression. Moreover, miR-455-5p mimic and SOCS3 knockdown significantly reversed the GAS5 overexpression-mediated suppression of the JAK2/STAT3 signaling and promotion of M1 polarization. CONCLUSION:GAS5 promotes M1 macrophage polarization by acting as a competing endogenous RNA of miR-455-5p to facilitate SOCS3 expression in childhood pneumonia.
Authors: Claudia Martini; Fernando Nicolas Sosa; Ricardo Malvicini; Natalia Pacienza; Gustavo Yannarelli; María Del C Vila Journal: J Physiol Biochem Date: 2021-07-24 Impact factor: 4.158