Molecular basis of the phenotypic characteristics of mammalian muscle fibres.

Adult mammalian skeletal muscle fibres can be separated into two distinct groups, fast and slow. Within each group there is a continuum of metabolic enzyme activity levels. In addition there are fast and slow isoforms of various myofibrillar proteins such as myosin, tropomyosin and troponin. These proteins are multimeric and multiple isoforms of their subunits assemble to create a continuum of subtypes within each major group. Fibres which coexpress both fast and slow subunit isoforms have an increased number of possible isoform combinations such that an entire spectrum of fibre 'types' is found between the two extremes, fast and slow. Numerous myosin heavy chain and fast troponin T isoforms further increase the diversity of muscle fibres. Such cellular diversity helps to explain the dynamic nature of skeletal muscle. Each individual fibre is able to respond to various functional demands by appropriate changes in its phenotypic expression of specific proteins.