| Literature DB >> 30583362 |
Deyun He1, Zhengzong Wu2, Bo Cui3, Zhengyu Jin4.
Abstract
We developed a novel aptasensor based on surface enhanced Raman spectroscopy (SERS) and applied it for highly sensitive detection of microcystin-LR (MC-LR). In this work, MC-LR aptamer and its corresponding complementary DNA fragments (cDNA) were conjugated to gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), respectively. Then, MC-LR aptamer-AuNPs and cDNA-MNPs conjugates were used as signal probes and capture probes, respectively. The proposed assay exhibited a linearity range from 0.01 to 200 ng/mL with the limit of detection (LOD) of 0.002 ng/mL. In addition, the reliability of the novel approach was validated by detecting different levels of MC-LR spiked in tap water samples. Overall, the novel aptasensor paves a new way for rapid and accurate determination of MC-LR and can be referred to detect other hazardous substances in water products.Entities:
Keywords: Food analysis; Magnetic nanoparticles; Microcystin-LR; Rapid detection; Surface enhanced Raman spectroscopy
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Year: 2018 PMID： 30583362 DOI： 10.1016/j.foodchem.2018.11.071
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514