Literature DB >> 3058328

Cosmids carrying Aspergillus terreus DNA can integrate into Saccharomyces cerevisiae chromosome XII via recombination between yeast and foreign DNAs.

E A Shoubochkina1, I I Fodor.   

Abstract

A genome clonotheque consisting of 25- to 40-kb Sau3AI fragments of Aspergillus terreus DNA was constructed in the episomal cosmid vector pES33 containing the yeast ARG4 gene. From the 475 transformants of cir0 yeast strain ESH-0, 23 stable Arg+ transformants were independently selected. Genetic and Southern analysis of these stable transformants showed that 39% arose as a result of recombination between cloned A. terreus DNA sequences and yeast chromosome XII. The recombination events most likely occurred in the regions of homology within the rDNA clusters of A. terreus and Saccharomyces cerevisiae.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3058328     DOI： 10.1007/bf00376738

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  26 in total

1.  Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of argininosuccinate lyase (argH) mutations.

Authors:  L Clarke; J Carbon
Journal:  J Mol Biol       Date:  1978-04-25       Impact factor: 5.469

2.  Rapid and efficient cosmid cloning.

Authors:  D Ish-Horowicz; J F Burke
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

3.  A small cosmid for efficient cloning of large DNA fragments.

Authors:  B Hohn; J Collins
Journal:  Gene       Date:  1980-11       Impact factor: 3.688

4.  A rapid chromosome-mapping method for cloned fragments of yeast DNA.

Authors:  S C Falco; D Botstein
Journal:  Genetics       Date:  1983-12       Impact factor: 4.562

5.  Genetic properties of chromosomally integrated 2 mu plasmid DNA in yeast.

Authors:  S C Falco; Y Li; J R Broach; D Botstein
Journal:  Cell       Date:  1982-06       Impact factor: 41.582

6.  Genetic map of Saccharomyces cerevisiae.

Authors:  R K Mortimer; D Schild
Journal:  Microbiol Rev       Date:  1980-12

7.  Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins.

Authors:  P J Schatz; L Pillus; P Grisafi; F Solomon; D Botstein
Journal:  Mol Cell Biol       Date:  1986-11       Impact factor: 4.272

8.  The isolation of nuclei from the filamentous fungus Aspergillus nidulans.

Authors:  M A Gealt; G Sheir-Neiss; N R Morris
Journal:  J Gen Microbiol       Date:  1976-05

9.  The nucleotide sequence of the yeast ARG4 gene.

Authors:  I R Beacham; B W Schweitzer; H M Warrick; J Carbon
Journal:  Gene       Date:  1984-09       Impact factor: 3.688

10.  Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity.

Authors:  S Smolik-Utlaut; T D Petes
Journal:  Mol Cell Biol       Date:  1983-07       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.