Literature DB >> 30582957

Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification.

Rong Zeng1, Jinyan Luo2, Shigang Gao3, Lihui Xu4, Zhiwei Song5, Fuming Dai6.   

Abstract

Reverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. CGMMV on inoculated leaves was tested using RT-RPA assay, suggesting that this method is suitable for CGMMV detection from leaves.
Copyright © 2018 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  CGMMV; Isothermal amplification; Specificity

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Substances：

Year:  2018        PMID： 30582957     DOI： 10.1016/j.mcp.2018.12.005

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  3 in total

1.  Reverse transcriptase loop-mediated isothermal amplification and reverse transcriptase recombinase amplification assays for rapid and sensitive detection of cardamom vein clearing virus.

Authors:  K P Naveen; A I Bhat
Journal:  3 Biotech       Date:  2020-05-12       Impact factor: 2.406

2.  Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus.

Authors:  Zhao-Hua Wang; Xiao-Jia Wang; Shao-Hua Hou
Journal:  J Virol Methods       Date:  2019-06-16       Impact factor: 2.014

3.  Detection of melon necrotic spot virus by one-step reverse transcription loop-mediated isothermal amplification assay.

Authors:  Ning Qiao; Huijie Dai; Jie Liu; Xiaoping Zhu; Jintang Li; Dezhen Zhang; Yongguang Liu
Journal:  PLoS One       Date:  2020-03-05       Impact factor: 3.240
  3 in total

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