Literature DB >> 3058122

Is there a tripeptidyl peptidase in the renal brush-border membrane?

A J Kenny1, J Ingram.   

Abstract

A recent claim that the renal brush border contains a tripeptidyl peptidase [Andersen & McDonald (1987) Am. J. Physiol. 253, F649-F655] was examined. In a fluorescent assay, the hydrolysis of Gly-Pro-Met-2-naphthylamide (-NH-Nap) and Gly-Pro-Leu-NH-Nap by pig kidney microvilli was strongly inhibited by amastatin or di-isopropyl phosphorofluoridate (inhibitors of aminopeptidases and dipeptidyl peptidase IV). The products formed were shown to be Gly-Pro and Met-NH-Nap (or Leu-NH-Nap) and free 2-naphthylamine. Specific antibodies to pig and rat aminopeptidase N abolished the apparent tripeptidyl peptidase activity. We conclude that these substrates are hydrolysed by the sequential attack of dipeptidyl peptidase IV and aminopeptidase N and that pig and rat brush borders lack a detectable tripeptidyl peptidase.

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Year:  1988        PMID： 3058122      PMCID： PMC1135234     

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  Release of a plasma membrane-bound triaminopeptidase activity from mammalian cells by thermolysin.

Authors:  T Aoyagi; H Suda; M Nagai; H Tobe; J Suzuki; T Takeuchi; H Umezawa
Journal:  Biochem Biophys Res Commun       Date:  1978-01-30       Impact factor: 3.575

2.  The metabolism of neuropeptides. Hydrolysis of peptides by the phosphoramidon-insensitive rat kidney enzyme 'endopeptidase-2' and by rat microvillar membranes.

Authors:  S L Stephenson; A J Kenny
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

3.  A rapid method for the preparation of microvilli from rabbit kidney.

Authors:  A G Booth; A J Kenny
Journal:  Biochem J       Date:  1974-09       Impact factor: 3.857

4.  Substance P and [Leu]enkephalin are hydrolyzed by an enzyme in pig caudate synaptic membranes that is identical with the endopeptidase of kidney microvilli.

Authors:  R Matsas; I S Fulcher; A J Kenny; A J Turner
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205

5.  Microvillar membrane neutral endopeptidases.

Authors:  A J Kenny; I S Fulcher; K Ridgwell; J Ingram
Journal:  Acta Biol Med Ger       Date:  1981

6.  Proteins of the kidney microvillar membrane. The 130 kDa protein in pig kidney, recognized by monoclonal antibody GK5C1, is an ectoenzyme with aminopeptidase activity.

Authors:  N S Gee; A J Kenny
Journal:  Biochem J       Date:  1985-09-15       Impact factor: 3.857

7.  Proteins of the kidney microvillar membrane. The amphipathic forms of endopeptidase purified from pig kidneys.

Authors:  I S Fulcher; A J Kenny
Journal:  Biochem J       Date:  1983-06-01       Impact factor: 3.857

8.  Partial purification and characterization of an ovarian tripeptidyl peptidase: a lysosomal exopeptidase that sequentially releases collagen-related (Gly-Pro-X) triplets.

Authors:  J K McDonald; A R Hoisington; D A Eisenhauer
Journal:  Biochem Biophys Res Commun       Date:  1985-01-16       Impact factor: 3.575

9.  Tripeptidyl aminopeptidase in the extralysosomal fraction of rat liver.

Authors:  R M Bålöw; U Ragnarsson; O Zetterqvist
Journal:  J Biol Chem       Date:  1983-10-10       Impact factor: 5.486

10.  Identification of a tripeptidyl aminopeptidase in the anterior pituitary gland: effect on the chemical and biological properties of rat and bovine growth hormones.

Authors:  T W Doebber; A R Divor; S Ellis
Journal:  Endocrinology       Date:  1978-11       Impact factor: 5.051
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