Literature DB >> 30580420

Cell-Free DNA: An Overview of Sample Types and Isolation Procedures.

Filippo Martignano1.   

Abstract

The study of cell-free DNA (cfDNA) is often challenging due to genomic DNA contamination, low concentration, and high fragmentation. Therefore, it is important to optimize pre-analytical and analytical procedures in order to maximize the performance of cfDNA-based analyses.In this chapter, we report the most common methods for the correct collection, centrifugation, storage, and DNA isolation from cell-free biological sources such as plasma, urines, cerebrospinal fluid, and pleural effusion fluid.

Entities:  

Keywords:  Cell-free DNA; Centrifugation; Cerebrospinal fluid; Collection; Nucleic acid extraction; Plasma; Pleural effusion fluid; Serum; Storage; Urines

Mesh：

Substances：

Year:  2019        PMID： 30580420     DOI： 10.1007/978-1-4939-8973-7_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Nanopore sequencing from liquid biopsy: analysis of copy number variations from cell-free DNA of lung cancer patients.

Authors:  Filippo Martignano; Uday Munagala; Stefania Crucitta; Alessandra Mingrino; Roberto Semeraro; Marzia Del Re; Iacopo Petrini; Alberto Magi; Silvestro G Conticello
Journal:  Mol Cancer       Date:  2021-02-12       Impact factor: 27.401

Review 2.  A clinician's handbook for using ctDNA throughout the patient journey.

Authors:  Samantha O Hasenleithner; Michael R Speicher
Journal:  Mol Cancer       Date:  2022-03-21       Impact factor: 27.401

Review 3.  Circulating Cell-Free DNA-Based Methylation Pattern in Saliva for Early Diagnosis of Head and Neck Cancer.

Authors:  Natalia Birknerova; Veronika Mancikova; Evan David Paul; Jan Matyasovsky; Pavol Cekan; Vladimir Palicka; Helena Parova
Journal:  Cancers (Basel)       Date:  2022-10-06       Impact factor: 6.575

4.  The Impact of Pre-transplant Cell-free DNA Levels on Leukemia Relapse and Transplant-related Complications in Allogeneic Hematopoietic Stem Cell Transplant Recipients

Authors:  Zeynep Arzu Yegin; Ferda Can; Sanem Gökçen; Rezzan Eren Sadioğlu; Zübeyde Nur Özkurt; Çiğdem İlhan; Münci Yağcı
Journal:  Balkan Med J       Date:  2020-01-23       Impact factor: 2.021

Review 5.  Circulating tumor DNA as an emerging liquid biopsy biomarker for early diagnosis and therapeutic monitoring in hepatocellular carcinoma.

Authors:  Xiaolin Wu; Jiahui Li; Asmae Gassa; Denise Buchner; Hakan Alakus; Qiongzhu Dong; Ning Ren; Ming Liu; Margarete Odenthal; Dirk Stippel; Christiane Bruns; Yue Zhao; Roger Wahba
Journal:  Int J Biol Sci       Date:  2020-03-05       Impact factor: 6.580

Review 6.  Technical and Methodological Aspects of Cell-Free Nucleic Acids Analyzes.

Authors:  Zuzana Pös; Ondrej Pös; Jakub Styk; Angelika Mocova; Lucia Strieskova; Jaroslav Budis; Ludevit Kadasi; Jan Radvanszky; Tomas Szemes
Journal:  Int J Mol Sci       Date:  2020-11-16       Impact factor: 5.923
  6 in total

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