Constance Delaby1, Laia Muñoz2, Soraya Torres2, Alícia Nadal3, Nathalie Le Bastard4, Sylvain Lehmann5, Alberto Lleó2, Daniel Alcolea6. 1. Université de Montpellier, CHU de Montpellier, Laboratoire de Biochimie-Protéomique clinique, INSERM U1183, Montpellier, France.; Sant Pau Memory Unit, Department of Neurology, Institut d'Investigacions Biomèdiques Sant Pau, Hospital de Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain. 2. Sant Pau Memory Unit, Department of Neurology, Institut d'Investigacions Biomèdiques Sant Pau, Hospital de Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas, CIBERNED, Spain. 3. Fujirebio Iberia, SLU, Barcelona, Spain. 4. Fujirebio Europe NV, Gent, Belgium. 5. Université de Montpellier, CHU de Montpellier, Laboratoire de Biochimie-Protéomique clinique, INSERM U1183, Montpellier, France. 6. Sant Pau Memory Unit, Department of Neurology, Institut d'Investigacions Biomèdiques Sant Pau, Hospital de Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas, CIBERNED, Spain. Electronic address: dalcolea@santpau.cat.
Abstract
OBJECTIVES: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. METHODS: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF Aβ1-42, Aβ1-40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio. RESULTS: Levels of CSF Aβ1-42 and Aβ1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio Aβ1-42/Aβ1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition. CONCLUSION: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.
OBJECTIVES: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. METHODS: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF Aβ1-42, Aβ1-40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio. RESULTS: Levels of CSF Aβ1-42 and Aβ1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio Aβ1-42/Aβ1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition. CONCLUSION: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.
Authors: Michelle R Campbell; Susan Ashrafzadeh-Kian; Ronald C Petersen; Michelle M Mielke; Jeremy A Syrjanen; Argonde C van Harten; Val J Lowe; Clifford R Jack; Joshua A Bornhorst; Alicia Algeciras-Schimnich Journal: Alzheimers Dement (Amst) Date: 2021-05-18
Authors: Daniel Alcolea; Jordi Pegueroles; Laia Muñoz; Valle Camacho; Diego López-Mora; Alejandro Fernández-León; Nathalie Le Bastard; Els Huyck; Alicia Nadal; Verónica Olmedo; Frederic Sampedro; Victor Montal; Eduard Vilaplana; Jordi Clarimón; Rafael Blesa; Juan Fortea; Alberto Lleó Journal: Ann Clin Transl Neurol Date: 2019-08-28 Impact factor: 4.511
Authors: Eline A J Willemse; Betty M Tijms; Bart N M van Berckel; Nathalie Le Bastard; Wiesje M van der Flier; Philip Scheltens; Charlotte E Teunissen Journal: Alzheimers Dement (Amst) Date: 2021-05-01
Authors: Ashvini Keshavan; Henrietta Wellington; Zhongbo Chen; Ayesha Khatun; Miles Chapman; Melanie Hart; David M Cash; William Coath; Thomas D Parker; Sarah M Buchanan; Sarah E Keuss; Matthew J Harris; Heidi Murray-Smith; Amanda Heslegrave; Nick C Fox; Henrik Zetterberg; Jonathan M Schott Journal: Alzheimers Dement (Amst) Date: 2020-09-13
Authors: Constance Delaby; Teresa Estellés; Nuole Zhu; Javier Arranz; Isabel Barroeta; María Carmona-Iragui; Ignacio Illán-Gala; Miguel Ángel Santos-Santos; Miren Altuna; Isabel Sala; M Belén Sánchez-Saudinós; Laura Videla; Sílvia Valldeneu; Andrea Subirana; Mireia Tondo; Francisco Blanco-Vaca; Sylvain Lehmann; Olivia Belbin; Rafael Blesa; Juan Fortea; Alberto Lleó; Daniel Alcolea Journal: Alzheimers Res Ther Date: 2022-02-01 Impact factor: 6.982