Bioaccumulation of antibiotics in crops under long-term manure application: Occurrence, biomass response and human exposure.

Long-term manure application gives rise to the uptake of antibiotics by plants and antibiotics subsequent entry into the food chain, representing an important alternative pathway for human exposure to antibiotics. The antibiotics can cause negative effects on crop growth and productivity. The bioaccumulation and translocation of 14 target antibiotics in peanuts (Arachis hypogaea L.) and their effects on peanut relative biomass in fields with long-term (≥15 years) manure application were studied. The results showed that all the target antibiotics were found in manures and rhizosphere soils, and most of them were found in all peanut tissues (roots, shells, kernels, stem, and leaves). The antibiotic concentrations in peanut tissues were varied with the characteristics of antibiotics in soils. Tetracyclines were the dominating antibiotic compounds in all peanut tissues, accounting for 61%-80% of total antibiotics due to their relatively high concentration in rhizosphere soil. Most tetracyclines and quinolones preferentially accumulated in the roots and translocated to other peanut tissues than sulfonamides and macrolides. Furthermore, the influence of antibiotics in soil and crops on relative biomass of crop tissues varied with tissues and antibiotic types. Antibiotics significantly inhibited the tissue relative biomass in most cases, although stimulation of some antibiotics to crop biomass was also observed. We found that 18.3% of the variance of the peanut relative biomass was explained by antibiotics in soils and tissues. The estimated threshold of daily intake values suggests that the consumption of peanut kernels grown in field conditions with long-term manure application presents a moderate risk to human health.