Literature DB >> 3057075

A novel two colour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells.

C Czerkinsky1, Z Moldoveanu, J Mestecky, L A Nilsson, O Ouchterlony.   

Abstract

A novel assay system has been developed which is based on the ELISPOT methodology and employs a combination of two immunoenzyme visualization systems yielding distinct colour products. This variation permits the simultaneous enumeration of two different types of cell secreting antigenically distinct products. Optimal conditions for the concurrent detection of human mononuclear cells secreting IgG or IgA antibodies are described.

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Year:  1988        PMID： 3057075     DOI： 10.1016/0022-1759(88)90306-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  41 in total

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Authors:  H S Chang; D A Sack
Journal:  Clin Diagn Lab Immunol       Date:  2001-05

Review 2.  The functions of cytokines and their uses in toxicology.

Authors:  J R Foster
Journal:  Int J Exp Pathol       Date:  2001-06       Impact factor: 1.925

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Authors:  F Qadri; G Jonson; Y A Begum; C Wennerås; M J Albert; M A Salam; A M Svennerholm
Journal:  Clin Diagn Lab Immunol       Date:  1997-07

Review 4.  Evaluation of events occurring at mucosal surfaces: techniques used to collect and analyze mucosal secretions and cells.

Authors:  Bruno Guy
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5.  Antibody-secreting cells in human peripheral blood after oral immunization with an inactivated enterotoxigenic Escherichia coli vaccine.

Authors:  C Wennerås; A M Svennerholm; C Ahrén; C Czerkinsky
Journal:  Infect Immun       Date:  1992-07       Impact factor: 3.441

6.  Distribution, persistence, and recall of serum and salivary antibody responses to peroral immunization with protein antigen I/II of Streptococcus mutans coupled to the cholera toxin B subunit.

Authors:  M W Russell; H Y Wu
Journal:  Infect Immun       Date:  1991-11       Impact factor: 3.441

7.  Antibody-producing cells in peripheral blood and salivary glands after oral cholera vaccination of humans.

Authors:  C Czerkinsky; A M Svennerholm; M Quiding; R Jonsson; J Holmgren
Journal:  Infect Immun       Date:  1991-03       Impact factor: 3.441

8.  Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood.

Authors:  Giulietta Saletti; Nicolas Çuburu; Jae Seung Yang; Ayan Dey; Cecil Czerkinsky
Journal:  Nat Protoc       Date:  2013-05-09       Impact factor: 13.491

9.  Differential expression of chemokine receptors on human IgA+ and IgG+ B cells.

Authors:  C Johansson; I Ahlstedt; S Furubacka; E Johnsson; W W Agace; M Quiding-Järbrink
Journal:  Clin Exp Immunol       Date:  2005-08       Impact factor: 4.330

10.  Intraperitoneal immunization of human subjects with tetanus toxoid induces specific antibody-secreting cells in the peritoneal cavity and in the circulation, but fails to elicit a secretory IgA response.

Authors:  C Lue; A W van den Wall Bake; S J Prince; B A Julian; M L Tseng; J Radl; C O Elson; J Mestecky
Journal:  Clin Exp Immunol       Date:  1994-05       Impact factor: 4.330
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