| Literature DB >> 30570720 |
Mariana Piuri1,2, Graham F Hatfull3.
Abstract
Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format.Keywords: Antibiotics; DST; Fluoromycobacteriophage; Mycobacteria; Reporter phage; Resistance
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Year: 2019 PMID： 30570720 DOI： 10.1007/978-1-4939-8940-9_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745