Maarten Broeren1, Rélana Nowacki2, Feico Halbertsma3, Nicolaas Arents4, Sebastiaan Zegers5. 1. Clinical Chemist, Máxima Medical Center, Postbox 7777, 5500, MB, Veldhoven, The Netherlands. 2. Pediatrician, Maastricht University Medical Center, Postbox 5800, 6202, AZ, Maastricht, The Netherlands. 3. Pediatrician, Máxima Medical Center, Postbox 7777, 5500, MB, Veldhoven, The Netherlands. F.Halbertsma@mmc.nl. 4. Clinical Microbiologist, Máxima Medical Center, Postbox 7777, 5500, MB, Veldhoven, The Netherlands. 5. Pediatrician, Máxima Medical Center, Postbox 7777, 5500, MB, Veldhoven, The Netherlands. b.zegers@mmc.nl.
Abstract
Diagnosing a urinary tract infection in children is often difficult due to non-specific symptoms and requires invasive and time-consuming procedures. Flow cytometry is a new and rapid method of analyzing urine to confirm or exclude UTIs. We have investigated the sensitivity and specificity of urine flow cytometry (Sysmex UF1000i) compared to conventional diagnostic techniques in a prospective study from January 1, 2014 until January 1, 2015. All children under 13 years of age with a suspicion of urinary tract infection were screened using both urine flow cytometry and urine culture. A urinary tract infection was defined as the combination of leukocyturia (≥ 25 leukocytes per μl) and a positive urine culture in the presence of clinical symptoms. A total number of 412 urine samples were collected, of which 63 cases (15.3%) were positive for a urinary tract infection. Receiver operating characteristic analysis showed an area under the curve of 0.97 (95% confidence interval h0.93-1.00) for the bacterial count. When using a cut-off value of 250 bacteria/μl in the presence of leukocyturia, the sensitivity for urinary tract infection is 0.97 with a negative predictive value of 97%, and the specificity is 0.91 with a positive predictive value of 90%. Conclusion: Flow cytometry-based bacterial and leukocyte count analysis is a time-efficient method of diagnosing or ruling out urinary tract infection in children, with a higher sensitivity and specificity than dipstick and microscopic analysis. What is known • Screening for urinary tract infections in children is difficult due to invasive and time-consuming procedures. • There is both over- and under-treatment of urinary tract infections due to the delays in accurate diagnosing. What is new • Flow cytometry is a rapid and accurate method to provide useful information in the diagnosis of urinary tract infection in children. When negative, flow cytometry can exclude urinary tract infection in children with a high degree of confidence. When flow cytometry is positive, the possibility of a urinary tract infection in children is increased.
Diagnosing a urinary tract infection in children is often difficult due to non-specific symptoms and requires invasive and time-consuming procedures. Flow cytometry is a new and rapid method of analyzing urine to confirm or exclude UTIs. We have investigated the sensitivity and specificity of urine flow cytometry (Sysmex UF1000i) compared to conventional diagnostic techniques in a prospective study from January 1, 2014 until January 1, 2015. All children under 13 years of age with a suspicion of urinary tract infection were screened using both urine flow cytometry and urine culture. A urinary tract infection was defined as the combination of leukocyturia (≥ 25 leukocytes per μl) and a positive urine culture in the presence of clinical symptoms. A total number of 412 urine samples were collected, of which 63 cases (15.3%) were positive for a urinary tract infection. Receiver operating characteristic analysis showed an area under the curve of 0.97 (95% confidence interval h0.93-1.00) for the bacterial count. When using a cut-off value of 250 bacteria/μl in the presence of leukocyturia, the sensitivity for urinary tract infection is 0.97 with a negative predictive value of 97%, and the specificity is 0.91 with a positive predictive value of 90%. Conclusion: Flow cytometry-based bacterial and leukocyte count analysis is a time-efficient method of diagnosing or ruling out urinary tract infection in children, with a higher sensitivity and specificity than dipstick and microscopic analysis. What is known • Screening for urinary tract infections in children is difficult due to invasive and time-consuming procedures. • There is both over- and under-treatment of urinary tract infections due to the delays in accurate diagnosing. What is new • Flow cytometry is a rapid and accurate method to provide useful information in the diagnosis of urinary tract infection in children. When negative, flow cytometry can exclude urinary tract infection in children with a high degree of confidence. When flow cytometry is positive, the possibility of a urinary tract infection in children is increased.
Authors: D S Lin; S H Huang; C C Lin; Y C Tung; T T Huang; N C Chiu; H A Koa; H Y Hung; C H Hsu; W S Hsieh; D I Yang; F Y Huang Journal: Pediatrics Date: 2000-02 Impact factor: 7.124
Authors: Hong Phuoc Duong; Karl Martin Wissing; Nathalie Tram; Georges Mascart; Philippe Lepage; Khalid Ismaili Journal: J Clin Microbiol Date: 2016-09-28 Impact factor: 5.948