Literature DB >> 3056918

Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.

H Gruber1, G Kern, P Gauss, L Gold.   

Abstract

The bacteriophage T4 dexA gene product is required during infection of Escherichia coli strains carrying a mutation in the optA gene. We purified the DexA protein from cells which overproduced the protein. The protein was assayed for nuclease activity on synthetic di- and oligonucleotide substrates of known sequence and secondary structure. Sequence and structure significantly affected nuclease activity. The properties of the enzyme may explain the requirement for the DexA protein during infection of optA mutant hosts.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3056918      PMCID： PMC211689          DOI： 10.1128/jb.170.12.5830-5836.1988

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  CUUCGG hairpins: extraordinarily stable RNA secondary structures associated with various biochemical processes.

Authors:  C Tuerk; P Gauss; C Thermes; D R Groebe; M Gayle; N Guild; G Stormo; Y d'Aubenton-Carafa; O C Uhlenbeck; I Tinoco
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

2.  Tandem duplications of the rII region of bacteriophage T4D.

Authors:  D H Parma; L J Ingraham; M Snyder
Journal:  Genetics       Date:  1972-07       Impact factor: 4.562

3.  Genetics and physiology of bacteriophage T4 3'-phosphatase: evidence for involvement of the enzyme in T4 DNA metabolism.

Authors:  R E Depew; N R Cozzarelli
Journal:  J Virol       Date:  1974-04       Impact factor: 5.103

4.  Deletion analysis of two nonessential regions of the T4 genome.

Authors:  T Homyk; J Weil
Journal:  Virology       Date:  1974-10       Impact factor: 3.616

5.  Degradation of cytosin-containing bacterial and bacteriophage DNA after infection of Escherichia coli B with bacteriophage T4D wild type and with mutants defective in genes 46, 47 and 56.

Authors:  E M Kutter; J S Wiberg
Journal:  J Mol Biol       Date:  1968-12       Impact factor: 5.469

6.  Origin of concatemeric T7 DNA.

Authors:  J D Watson
Journal:  Nat New Biol       Date:  1972-10-18

7.  Function of the bacteriophage T4 transfer RNA's.

Authors:  J H Wilson
Journal:  J Mol Biol       Date:  1973-03-15       Impact factor: 5.469

8.  Separation and characterization of deoxyribonucleases of Escherichia coli B. II. Further purification and properties of an exonuclease induced by infection with bacteriophage T2.

Authors:  E C Short; J F Koerner
Journal:  J Biol Chem       Date:  1969-03-25       Impact factor: 5.157

9.  Gene 1.2 protein of bacteriophage T7. Effect on deoxyribonucleotide pools.

Authors:  J A Myers; B B Beauchamp; C C Richardson
Journal:  J Biol Chem       Date:  1987-04-15       Impact factor: 5.157

10.  A unique deoxyguanosine triphosphatase is responsible for the optA1 phenotype of Escherichia coli.

Authors:  B B Beauchamp; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205
View more
  4 in total

Review 1.  Bacteriophage T4 genome.

Authors:  Eric S Miller; Elizabeth Kutter; Gisela Mosig; Fumio Arisaka; Takashi Kunisawa; Wolfgang Rüger
Journal:  Microbiol Mol Biol Rev       Date:  2003-03       Impact factor: 11.056

2.  Intron homing with limited exon homology. Illegitimate double-strand-break repair in intron acquisition by phage t4.

Authors:  M M Parker; M Belisle; M Belfort
Journal:  Genetics       Date:  1999-12       Impact factor: 4.562

3.  Role of exonucleolytic degradation in group I intron homing in phage T4.

Authors:  Y J Huang; M M Parker; M Belfort
Journal:  Genetics       Date:  1999-12       Impact factor: 4.562

4.  The proofreading domain of Escherichia coli DNA polymerase I and other DNA and/or RNA exonuclease domains.

Authors:  M J Moser; W R Holley; A Chatterjee; I S Mian
Journal:  Nucleic Acids Res       Date:  1997-12-15       Impact factor: 16.971
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.