Lénia Rodrigues1, Rosa Espanca2, Ana Rodrigues Costa3, Célia Miguel Antunes3, Clarinda Pomar4, Fernando Capela-Silva5, Cristina Conceição Pinheiro6, Pedro Domingues7, Francisco Amado8, Elsa Lamy9. 1. Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM), University of Évora, Évora, Portugal. 2. Grouping of Health Centres of Central Alentejo (ACES, AC), Évora, Portugal. 3. Institute of Earth Sciences (ICT), Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM) and Department of Chemistry, University of Évora, Évora, Portugal. 4. Centre of Research in Education and Psychology (CIEP) and Department of Pedagogy and Education, University of Évora, Évora, Portugal. 5. Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM) and Department of Biology, University of Évora, Évora, Portugal. 6. Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM) and Department of Zootechnics, University of Évora, Évora, Portugal. 7. Organic Chemistry, Natural and Agrofood Products (QOPNA) and Department of Chemistry, University of Aveiro, Aveiro, Portugal. 8. Organic Chemistry, Natural and Agrofood Products (QOPNA) and Department of Chemistry, University of Aveiro, Aveiro, Portugal. famado@ua.pt. 9. Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM), University of Évora, Évora, Portugal. ecsl@uevora.pt.
Abstract
BACKGROUND/ OBJECTIVES: Oral sensorial perception is a key aspect in food choices and knowing the mechanisms modulating such perception is of major importance in the context of child obesity, which is reaching high rates in Mediterranean countries. Salivary proteome has been linked to taste sensitivity in adults. The aim of this study was to search for differences in salivary proteomes of children with different bitter or sweet taste sensitivities and to assess if these potential differences are associated with their body mass index percentile (BMI percentile). SUBJECTS/ METHODS: 387 children aged 8-9 years old were assessed for BMI percentile and classified according to their sensitivity to bitter and sweet tastes, according to their caffeine and sucrose detection thresholds, respectively. Saliva protein composition was compared among taste sensitivity groups, taking into account BMI percentile and gender, using gel-based proteomics approaches, coupled to mass spectrometry for protein identification. RESULTS: Among the salivary proteins related to bitter taste sensitivity, higher levels of cystatins were observed in bitter-sensitive children, in the case of those of normal weight, and in bitter low-sensitive, in the case of overweight children. For sweetness, the relationship between saliva and taste perception was also dependent on BMI percentile, with several proteins (including salivary cystatins) differing between taste sensitivity groups, with disparities arising between normal-weight and overweight children. Cystatin isoforms A, B and SA were observed to be considerably increased in saliva from obese children. CONCLUSIONS: Salivary proteome is related with sensitivities to bitter and sweet tastes in children, but the association is dependent on BMI percentile and gender.
BACKGROUND/ OBJECTIVES: Oral sensorial perception is a key aspect in food choices and knowing the mechanisms modulating such perception is of major importance in the context of childobesity, which is reaching high rates in Mediterranean countries. Salivary proteome has been linked to taste sensitivity in adults. The aim of this study was to search for differences in salivary proteomes of children with different bitter or sweet taste sensitivities and to assess if these potential differences are associated with their body mass index percentile (BMI percentile). SUBJECTS/ METHODS: 387 children aged 8-9 years old were assessed for BMI percentile and classified according to their sensitivity to bitter and sweet tastes, according to their caffeine and sucrose detection thresholds, respectively. Saliva protein composition was compared among taste sensitivity groups, taking into account BMI percentile and gender, using gel-based proteomics approaches, coupled to mass spectrometry for protein identification. RESULTS: Among the salivary proteins related to bitter taste sensitivity, higher levels of cystatins were observed in bitter-sensitive children, in the case of those of normal weight, and in bitter low-sensitive, in the case of overweight children. For sweetness, the relationship between saliva and taste perception was also dependent on BMI percentile, with several proteins (including salivary cystatins) differing between taste sensitivity groups, with disparities arising between normal-weight and overweight children. Cystatin isoforms A, B and SA were observed to be considerably increased in saliva from obesechildren. CONCLUSIONS: Salivary proteome is related with sensitivities to bitter and sweet tastes in children, but the association is dependent on BMI percentile and gender.
Authors: Fabiola Carolina Espinosa-Gómez; Eliel Ruíz-May; Juan Carlos Serio-Silva; Colin A Chapman Journal: PeerJ Date: 2020-07-22 Impact factor: 2.984
Authors: Elsa Lamy; Camila P Rubio; Laura Carreira; Fernando Capela E Silva; Silvia Martinez-Subiela; Fernando Tecles; Pia Lopez-Jornet; Jose J Ceron; Asta Tvarijonaviciute Journal: Sci Rep Date: 2022-06-08 Impact factor: 4.996