Literature DB >> 3056803

Optimal conditions for supercoil DNA sequencing with the Escherichia coli DNA polymerase I large fragment.

H M Lim1, J J Pène.   

Abstract

Sequencing ladders produced from supercoiled DNA templates with the Escherichia coli DNA polymerase Klenow fragment are often unreadable because of a high background and misincorporated nucleotides. This study showed that contaminating RNA molecules can interfere with template:primer hybridization. Procedures are provided for the purification of template DNA and stringent conditions for primer-template hybridization that overcome these problems.

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Year:  1988        PMID： 3056803     DOI： 10.1016/0735-0651(88)90024-6

Source DB:  PubMed          Journal:  Gene Anal Tech        ISSN： 0735-0651


  15 in total

Review 1.  Demystified ... DNA nucleotide sequencing.

Authors:  J S Randhawa; A J Easton
Journal:  Mol Pathol       Date:  1999-06

2.  Evolution of collagen IV genes from a 54-base pair exon: a role for introns in gene evolution.

Authors:  G Butticè; P Kaytes; J D'Armiento; G Vogeli; M Kurkinen
Journal:  J Mol Evol       Date:  1990-06       Impact factor: 2.395

3.  Sequence and expression of the Escherichia coli K1 neuC gene product.

Authors:  G Zapata; J M Crowley; W F Vann
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

4.  The exon trapping assay partly discriminates against alternatively spliced exons.

Authors:  A Andreadis; P E Nisson; K S Kosik; P C Watkins
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971

5.  In vivo assay of protein-protein interactions in Hin-mediated DNA inversion.

Authors:  S Y Lee; H J Lee; H Lee; S Kim; E H Cho; H M Lim
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

6.  Processing and antigenicity of entire and anchor-free spike glycoprotein S of coronavirus TGEV expressed by recombinant baculovirus.

Authors:  M Godet; D Rasschaert; H Laude
Journal:  Virology       Date:  1991-12       Impact factor: 3.616

7.  Direct neutralization of alkaline-denatured plasmid DNA in sequencing protocol by the sequencing reagent itself.

Authors:  E Rouer
Journal:  Nucleic Acids Res       Date:  1994-11-11       Impact factor: 16.971

8.  Nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment protein G of bovine respiratory syncytial virus is distinct from that of human respiratory syncytial virus.

Authors:  R A Lerch; K Anderson; G W Wertz
Journal:  J Virol       Date:  1990-11       Impact factor: 5.103

9.  Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes.

Authors:  P A Bain; M Yoo; T Clarke; S H Hammond; A H Payne
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-15       Impact factor: 11.205

10.  Site-directed mutagenesis of dicarboxylic acids near the active site of Bacillus cereus 5/B/6 beta-lactamase II.

Authors:  H M Lim; R K Iyer; J J Pène
Journal:  Biochem J       Date:  1991-06-01       Impact factor: 3.857
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