Literature DB >> 30566524

Correction: Generation of murine tumor cell lines deficient in MHC molecule surface expression using the CRISPR/Cas9 system.

Krishna Das, David Eisel, Clarissa Lenkl, Ashish Goyal, Sven Diederichs, Elke Dickes, Wolfram Osen, Stefan B Eichmüller.   

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0174077.].

Entities:  

Year:  2018        PMID： 30566524      PMCID： PMC6300243          DOI： 10.1371/journal.pone.0209719

Source DB:  PubMed          Journal:  PLoS One        ISSN： 1932-6203            Impact factor:   3.240


There are two errors in S2 Table. Labeling of primer sequences for PCR amplification are incorrect. Primers given for IAbβ-chain are specific for β2m and vice versa. Furthermore, there is a mistake in the sequence for the nested forward primer specific for β2m.
S2 Table

Primers used to for mutation analysis at genomic target sites.

IAbβ-chainforward5'-TTTGCTTTCTGAAGGGGGCA-3'
reverse5'-AGAATGGAGTCTCACTCTCTCTT-3'
β2mnested forward5'-TCTGAGTGGGATATTGTCAGC-3'
nested reverse5'-TTGCGCTCAGGGAGTCTA-3'
forward5'-AAGGGTTGAGTTCTGCCAGTT-3'
reverse5'-TCTCTCGACTTCGGTTGGAT-3'
  1 in total

1.  Generation of murine tumor cell lines deficient in MHC molecule surface expression using the CRISPR/Cas9 system.

Authors:  Krishna Das; David Eisel; Clarissa Lenkl; Ashish Goyal; Sven Diederichs; Elke Dickes; Wolfram Osen; Stefan B Eichmüller
Journal:  PLoS One       Date:  2017-03-16       Impact factor: 3.240
  1 in total

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