Literature DB >> 30566515

Correction: A vesicular Na+/Ca2+ exchanger in coral calcifying cells.

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Abstract

[This corrects the article DOI: 10.1371/journal.pone.0205367.].

Entities:  

Year:  2018        PMID: 30566515      PMCID: PMC6300259          DOI: 10.1371/journal.pone.0209734

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


Fig 3 is incorrect. The authors have provided a corrected version here. The publisher apologizes for the error.
Fig 3

Validation of antibodies against AyNCXA.

A) The anti-AyNCXA antibodies recognize a ~100 kDa and ~75 kDa protein in homogenized A. yongei tissue. Both bands are eliminated when the antibody is pre-absorbed with the epitope peptide overnight, and neither band is present when the membrane is incubated with the pre-immune serum. All sample wells contain the same amount of protein and all three Western Blot images were taken at the same exposure. B) Immunofluorescence microscopy of A. yongei tissue reveals AyNCXA is present in all four tissue layers, including the calicodermis. C) Pre-absorption of antibodies with antigen peptide eliminates signal at the same exposure, confirming antibody specificity.

Validation of antibodies against AyNCXA.

A) The anti-AyNCXA antibodies recognize a ~100 kDa and ~75 kDa protein in homogenized A. yongei tissue. Both bands are eliminated when the antibody is pre-absorbed with the epitope peptide overnight, and neither band is present when the membrane is incubated with the pre-immune serum. All sample wells contain the same amount of protein and all three Western Blot images were taken at the same exposure. B) Immunofluorescence microscopy of A. yongei tissue reveals AyNCXA is present in all four tissue layers, including the calicodermis. C) Pre-absorption of antibodies with antigen peptide eliminates signal at the same exposure, confirming antibody specificity.
  1 in total

1.  A vesicular Na+/Ca2+ exchanger in coral calcifying cells.

Authors:  Megan E Barron; Angus B Thies; Jose A Espinoza; Katie L Barott; Amro Hamdoun; Martin Tresguerres
Journal:  PLoS One       Date:  2018-10-31       Impact factor: 3.240

  1 in total

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