| Literature DB >> 30555571 |
Kuan Hu1, Feng Yin1, Mengyin Yu1, Chengjie Sun1, Jingxu Li1, Yujie Liang1, Wenjun Li1, Mingsheng Xie1, Yuanzhi Lao2, Wei Liang3, Zi-Gang Li1.
Abstract
[This corrects the article DOI: 10.7150/thno.19840.].Entities:
Year: 2018 PMID: 30555571 PMCID: PMC6276290 DOI: 10.7150/thno.31152
Source DB: PubMed Journal: Theranostics ISSN: 1838-7640 Impact factor: 11.556
Figure 3(A, B) MeR/PhR stabilize p53 and elevate protein levels of p53 and its targets MDM2 and MDMX. Log-phase PA-1 and MCF-7 cells were incubated with 40 μM peptides (MeR/S, PhR/S) or 1 μM nutlin-3a, and cell lysates were analyzed by Western blotting. (C, D) PhR shows induction of p53 and p53 target genes in p53wt cell lines. Exponentially growing p53wt cancer cell lines were incubated with 40 μM peptides or 1 μM nutlin-3a for 48 h, and mRNA level of p53 and p53 targets MDM2, MDMX, and MIC or stemness-related genes were analyzed by quantitative PCR and expressed as fold increase. Error bars represent SEMs for triplicates of three independent experiments, **, P<0.01. *, P<0.05.