Ingvild L Tangen1, Kristine E Fasmer2, Gonda F Konings3, Arthur Jochems4, Bert Delvoux3, Sofia Xanthoulea3, Tomasz Stokowy5, Elin Strand1, Hege F Berg1, Seppo Auriola6, Jone Trovik1, Merja R Häkkinen6, Ingfrid S Haldorsen2, Camilla Krakstad7, Andrea Romano3. 1. Department of Gynaecology and Obstetrics, Haukeland University Hospital, Bergen, Norway; Centre for Cancer Biomarkers CCBIO, Department of Clinical Science, University of Bergen, Bergen, Norway. 2. Mohn Medical Imaging and Visualization Centre, Department of Radiology, Haukeland University Hospital, Bergen, Norway; Section for Radiology, Department of Clinical Medicine, University of Bergen, Bergen, Norway. 3. Department of Gynaecology and Obstetrics, GROW - School for Oncology & Developmental Biology, Maastricht University, Maastricht, the Netherlands. 4. Department of Gynaecology and Obstetrics, GROW - School for Oncology & Developmental Biology, Maastricht University, Maastricht, the Netherlands; The D-Lab: Decision Support for Precision Medicine, GROW - School for Oncology and Developmental Biology, Maastricht University Medical Centre, Universiteitssingel 40, 6229, ER, Maastricht, The Netherlands. 5. Department of Clinical Science, University of Bergen, Bergen, Norway. 6. School of Pharmacy, University of Eastern Finland, Kuopio, Finland. 7. Department of Gynaecology and Obstetrics, Haukeland University Hospital, Bergen, Norway; Centre for Cancer Biomarkers CCBIO, Department of Clinical Science, University of Bergen, Bergen, Norway. Electronic address: camilla.krakstad@uib.no.
Abstract
BACKGROUND: Despite being a hormone dependent cancer, there is limited knowledge regarding the relation between level of steroids in blood and prognosis for endometrial cancer (EC) patients. METHODS: In this study we investigated plasma levels of 19 steroids using liquid-chromatography tandem mass-spectrometry in 38 postmenopausal EC patients, 19 with long, and 19 with short survival. We explored if estradiol levels were associated with specific abdominal fat distribution patterns and if transcriptional alterations related to estradiol levels could be observed in tumor samples. RESULTS: The plasma steroid levels for DHEA, DHEAS, progesterone, 21 OH progesterone and E1S were significantly increased (all p < 0.05) in patients with long survival compared to short. Estradiol levels were significantly positively correlated with visceral fat percentage (p = 0.035), and an increased expression of genes involved in estrogen related signaling was observed in tumors from patients with high estradiol levels in plasma. CONCLUSION: Several of the identified plasma steroids represent promising biomarkers in EC patients. The association between increased estradiol levels and a high percentage of visceral fat indicates that visceral fat is a larger contributor to estradiol production compared to subcutaneous fat in this population.
BACKGROUND: Despite being a hormone dependent cancer, there is limited knowledge regarding the relation between level of steroids in blood and prognosis for endometrial cancer (EC) patients. METHODS: In this study we investigated plasma levels of 19 steroids using liquid-chromatography tandem mass-spectrometry in 38 postmenopausal EC patients, 19 with long, and 19 with short survival. We explored if estradiol levels were associated with specific abdominal fat distribution patterns and if transcriptional alterations related to estradiol levels could be observed in tumor samples. RESULTS: The plasma steroid levels for DHEA, DHEAS, progesterone, 21 OH progesterone and E1S were significantly increased (all p < 0.05) in patients with long survival compared to short. Estradiol levels were significantly positively correlated with visceral fat percentage (p = 0.035), and an increased expression of genes involved in estrogen related signaling was observed in tumors from patients with high estradiol levels in plasma. CONCLUSION: Several of the identified plasma steroids represent promising biomarkers in EC patients. The association between increased estradiol levels and a high percentage of visceral fat indicates that visceral fat is a larger contributor to estradiol production compared to subcutaneous fat in this population.
Authors: Linda Stevens Brentjens; Denise Habets; Janneke Den Hartog; Salwan Al-Nasiry; Lotte Wieten; Servaas Morré; Aafke Van Montfoort; Andrea Romano; Ron van Golde Journal: BMJ Open Date: 2022-06-08 Impact factor: 3.006
Authors: Elin Strand; Ingvild L Tangen; Kristine E Fasmer; Havjin Jacob; Mari K Halle; Erling A Hoivik; Bert Delvoux; Jone Trovik; Ingfrid S Haldorsen; Andrea Romano; Camilla Krakstad Journal: Metabolites Date: 2019-12-14