Jin Huang1, Jun-Jie Hang2, Xiang-Rong Qin1, Jian Huang1, Xiao-Yong Wang3. 1. Department of Gastroenterology, Changzhou No. 2 People's Hospital, Xinglong Xiang 29, Changzhou, 213000, Jiangsu, China. 2. Department of Oncology, Changzhou No. 2 People's Hospital, Xinglong Xiang 29, Changzhou, 213000, China. 3. Department of Gastroenterology, Changzhou No. 2 People's Hospital, Xinglong Xiang 29, Changzhou, 213000, Jiangsu, China. wxy20009@126.com.
Abstract
BACKGROUND: Genetic polymorphisms of Toll-like receptors play important roles in gastric carcinogenesis. The aim of this study was to determine the role of TLR2-196 to -174 ins/del polymorphism in gastric cancer susceptibility and prognosis. METHODS: This study included 520 people from southern China. Samples were genotyped by the allele-specific polymerase chain reaction, among which 10% were randomly selected for sequencing. The serological method was used to determine Helicobacter pylori. RESULTS: The TLR2 genotype was not associated with the risk of H. pylori infection. The del/del genotype exhibited significantly higher gastric cancer risk (adjusted OR 2.59, 95% CI 1.33‒5.07) than that of the ins/ins genotype. Further stratification analyses demonstrated that the del/del genotype was associated with a risk of intestinal gastric cancer (adjusted OR 2.62, 95% CI 1.34-5.14). In addition, the presence of the del/del genotype and the H. pylori infection conferred a synergistic effect (OR 3.04, 95% CI 1.33‒6.98) for the development of gastric cancer. The del/del genotype was not associated with a poor prognosis in gastric cancer patients. CONCLUSION: The del/del genotype is associated with an increased gastric cancer risk in the southern Chinese population. However, TLR2 polymorphism is neither associated with H. pylori infection, nor with a poor prognosis.
BACKGROUND: Genetic polymorphisms of Toll-like receptors play important roles in gastric carcinogenesis. The aim of this study was to determine the role of TLR2-196 to -174 ins/del polymorphism in gastric cancer susceptibility and prognosis. METHODS: This study included 520 people from southern China. Samples were genotyped by the allele-specific polymerase chain reaction, among which 10% were randomly selected for sequencing. The serological method was used to determine Helicobacter pylori. RESULTS: The TLR2 genotype was not associated with the risk of H. pyloriinfection. The del/del genotype exhibited significantly higher gastric cancer risk (adjusted OR 2.59, 95% CI 1.33‒5.07) than that of the ins/ins genotype. Further stratification analyses demonstrated that the del/del genotype was associated with a risk of intestinal gastric cancer (adjusted OR 2.62, 95% CI 1.34-5.14). In addition, the presence of the del/del genotype and the H. pyloriinfection conferred a synergistic effect (OR 3.04, 95% CI 1.33‒6.98) for the development of gastric cancer. The del/del genotype was not associated with a poor prognosis in gastric cancerpatients. CONCLUSION: The del/del genotype is associated with an increased gastric cancer risk in the southern Chinese population. However, TLR2 polymorphism is neither associated with H. pyloriinfection, nor with a poor prognosis.
Entities:
Keywords:
Gastric cancer; H. pylori; Polymorphism; Prognosis; TLR2
Authors: Caroline de Matos Lourenço; Manoela Dias Susi; Mariah Cristina Antunes do Nascimento; Vilson Serafim Junior; Ana Paula Simedan Vila; Gabriela Helena Rodrigues-Flemming; Eny Maria Goloni-Bertollo; Ana Elizabete Silva; Juliana Garcia de Oliveira-Cucolo Journal: World J Gastrointest Oncol Date: 2020-05-15