Cátia Caneiras1, Luís Lito2, Sagrario Mayoralas-Alises3, Salvador Díaz-Lobato4, José Melo-Cristino5, Aida Duarte6. 1. Microbiology and Immunology Department, Interdisciplinary Research Centre Egas Moniz (CiiEM), Faculty of Pharmacy, University of Lisbon, 1649-003, Lisbon, Portugal; Institute of Environmental Health (ISAMB), Faculty of Medicine, University of Lisbon, 1649-028, Lisbon, Portugal. Electronic address: ccaneiras@gmail.com. 2. Laboratory of Microbiology, Centro Hospitalar Lisboa Norte, 1649-035, Lisbon, Portugal. 3. Pneumological Department, Moncloa University Hospital, 28008, Madrid, Spain; European University, 28108, Alcobendas, Madrid, Spain. 4. Pneumological Department, Ramón y Cajal University Hospital, Madrid, Spain; Institute Ramón y Cajal for Health Research (IRYCIS), Alcalá de Henares University, 28034, Madrid, Spain. 5. Laboratory of Microbiology, Centro Hospitalar Lisboa Norte, 1649-035, Lisbon, Portugal; Institute of Microbiology, Faculty of Medicine, University of Lisbon, 1649-028, Lisbon, Portugal; Institute of Microbiology, Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, Lisbon, Portugal. 6. Microbiology and Immunology Department, Interdisciplinary Research Centre Egas Moniz (CiiEM), Faculty of Pharmacy, University of Lisbon, 1649-003, Lisbon, Portugal.
Abstract
INTRODUCTION: The rapid and complex evolution of bacterial resistance mechanisms in Klebsiella pneumoniae producing extended-spectrum β-lactamases and carbapenemases in Klebsiella pneumoniae is one of the most significant threats to public health. However, questions and controversies regarding the interactions between resistance and virulence in multidrug-resistant K. pneumoniae isolates remain unclear. METHODS: A retrospective cohort study was performed with 100 K. pneumoniae isolates recovered from a tertiary care university hospital centre in Lisbon over a 31-year period. Resistance and virulence determinants were screened using molecular methods (PCR, M13-PCR and MLST). RESULTS: The predominant virulence profile (fimH, mrkDv1, khe) was shared by all isolates, indicative of an important role of type 1 and 3 fimbrial adhesins and haemolysin, regardless of the type of β-lactamase produced. However, accumulation of virulence factors was identified in KPC-3-producers, with a higher frequency (p<0.05) of capsular serotype K2 and iucC aerobactin when compared with non-KPC-3 β-lactamases or carbapenemases. Additionally, 9 different virulence profiles were found, indicating that the KPC-3 carbapenemase producers seem to adapt successfully to the host environment and maintain virulence via several pathways. CONCLUSION: This study describes an overlapping of multidrug-resistance and virulence determinants in ST-14K2 KPC-3 K. pneumoniae clinical isolates that may impose an additional challenge in the treatment of infections caused by this pathogen.
INTRODUCTION: The rapid and complex evolution of bacterial resistance mechanisms in Klebsiella pneumoniae producing extended-spectrum β-lactamases and carbapenemases in Klebsiella pneumoniae is one of the most significant threats to public health. However, questions and controversies regarding the interactions between resistance and virulence in multidrug-resistant K. pneumoniae isolates remain unclear. METHODS: A retrospective cohort study was performed with 100 K. pneumoniae isolates recovered from a tertiary care university hospital centre in Lisbon over a 31-year period. Resistance and virulence determinants were screened using molecular methods (PCR, M13-PCR and MLST). RESULTS: The predominant virulence profile (fimH, mrkDv1, khe) was shared by all isolates, indicative of an important role of type 1 and 3 fimbrial adhesins and haemolysin, regardless of the type of β-lactamase produced. However, accumulation of virulence factors was identified in KPC-3-producers, with a higher frequency (p<0.05) of capsular serotype K2 and iucC aerobactin when compared with non-KPC-3 β-lactamases or carbapenemases. Additionally, 9 different virulence profiles were found, indicating that the KPC-3 carbapenemase producers seem to adapt successfully to the host environment and maintain virulence via several pathways. CONCLUSION: This study describes an overlapping of multidrug-resistance and virulence determinants in ST-14K2 KPC-3K. pneumoniae clinical isolates that may impose an additional challenge in the treatment of infections caused by this pathogen.
Authors: Gabriel Mendes; João F Ramalho; Ana Bruschy-Fonseca; Luís Lito; Aida Duarte; José Melo-Cristino; Cátia Caneiras Journal: Antibiotics (Basel) Date: 2022-01-27
Authors: João Perdigão; Cátia Caneiras; Rita Elias; Ana Modesto; Anton Spadar; Jody Phelan; Susana Campino; Taane G Clark; Eliana Costa; Maria José Saavedra; Aida Duarte Journal: Microorganisms Date: 2020-12-13
Authors: Gabriel Mendes; João F Ramalho; Ana Bruschy-Fonseca; Luís Lito; Aida Duarte; José Melo-Cristino; Cátia Caneiras Journal: Microorganisms Date: 2022-02-11
Authors: Marta Aires-de-Sousa; José Manuel Ortiz de la Rosa; Maria Luísa Gonçalves; Ana Luísa Pereira; Patrice Nordmann; Laurent Poirel Journal: Emerg Infect Dis Date: 2019-09 Impact factor: 6.883