Paola Illesca1, Rodrigo Valenzuela2, Alejandra Espinosa3, Francisca Echeverría4, Sandra Soto-Alarcon4, Macarena Ortiz5, Luis A Videla6. 1. Biochemistry Department, Faculty of Biochemistry, University of Litoral, Santa Fe, Argentina. 2. Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. Electronic address: rvalenzuelab@med.uchile.cl. 3. Medical Technology Department, Faculty of Medicine, University of Chile, Santiago, Chile. 4. Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. 5. Nutriton and Dietetics School, Faculty of Health Sciences, Catholic University of Maule, Curicó, Chile. 6. Molecular and Clinical Pharmacology Program, Institute of Biomedical Science, Faculty of Medicine, University of Chile, Santiago, Chile.
Abstract
BACKGROUND: White adipose tissue (WAT) have a relevant metabolic and inflammatory function, in overweight or obesity conditions. In this regard, the WAT under over feeding nutrition present a significant increment in oxidative stress, pro-inflammatory status and depletion of n-3 long chain polyunsaturated fatty acid. Hydroxytyrosol (HT) is a polyphenol with important cytoprotective effects, and this molecule can modulate the gene expression, transcription factors and enzymatic activity. OBJECTIVE: Therefore, the purpose of this study was evaluate the anti-inflammatory, anti-oxidant and anti-lipogenic effects of HT supplementation mice and the molecular adaptations involved, on dysfunctional WAT from high-fat diet (HFD)-fed mice. METHODS AND RESULTS: Male C57BL/6 J mice received (i) control diet (10% fat); (ii) control diet + HT (daily doses of 5 mg kg body weight), (iii) HFD (60% fat); or (iv) HFD + HT for 12 weeks. HFD-fed mice exhibited: (i) WAT hypertrophy; (ii) oxidative stress and depletion of antioxidant defenses, (iii) increased lipogenesis and pro-inflammatory status, (iv) depletion of n-3 LCPUFA and (v) up-regulation of NF-κB and SREBP 1c with down-regulation Nrf2, and PPAR-γ. HT supplementation attenuated the metabolic impairment produced by HFD in WAT, attenuating increment of NF-κB and SREBP 1c, and increasing the activity of Nrf2 and PPAR-γ. CONCLUSION: Supplementation with HT improve the WAT dysfunction induced by HDF in mice through the modulation of transcription factors NF-κB, Nrf2, SREBP-1c and PPAR-γ as well as their target genes, involved in inflammation, antioxidant defenses and lipogenesis.
BACKGROUND: White adipose tissue (WAT) have a relevant metabolic and inflammatory function, in overweight or obesity conditions. In this regard, the WAT under over feeding nutrition present a significant increment in oxidative stress, pro-inflammatory status and depletion of n-3 long chain polyunsaturated fatty acid. Hydroxytyrosol (HT) is a polyphenol with important cytoprotective effects, and this molecule can modulate the gene expression, transcription factors and enzymatic activity. OBJECTIVE: Therefore, the purpose of this study was evaluate the anti-inflammatory, anti-oxidant and anti-lipogenic effects of HT supplementation mice and the molecular adaptations involved, on dysfunctional WAT from high-fat diet (HFD)-fed mice. METHODS AND RESULTS: Male C57BL/6 J mice received (i) control diet (10% fat); (ii) control diet + HT (daily doses of 5 mg kg body weight), (iii) HFD (60% fat); or (iv) HFD + HT for 12 weeks. HFD-fed mice exhibited: (i) WAT hypertrophy; (ii) oxidative stress and depletion of antioxidant defenses, (iii) increased lipogenesis and pro-inflammatory status, (iv) depletion of n-3 LCPUFA and (v) up-regulation of NF-κB and SREBP 1c with down-regulation Nrf2, and PPAR-γ. HT supplementation attenuated the metabolic impairment produced by HFD in WAT, attenuating increment of NF-κB and SREBP 1c, and increasing the activity of Nrf2 and PPAR-γ. CONCLUSION: Supplementation with HT improve the WAT dysfunction induced by HDF in mice through the modulation of transcription factors NF-κB, Nrf2, SREBP-1c and PPAR-γ as well as their target genes, involved in inflammation, antioxidant defenses and lipogenesis.
Authors: Cesar Ramirez-Tortosa; Ana Sanchez; Cristina Perez-Ramirez; Jose Luis Quiles; María Robles-Almazan; Mario Pulido-Moran; Pedro Sanchez-Rovira; MCarmen Ramirez-Tortosa Journal: Antioxidants (Basel) Date: 2019-09-11
Authors: Bright Asare-Bediako; Sunil K Noothi; Sergio Li Calzi; Baskaran Athmanathan; Cristiano P Vieira; Yvonne Adu-Agyeiwaah; Mariana Dupont; Bryce A Jones; Xiaoxin X Wang; Dibyendu Chakraborty; Moshe Levi; Prabhakara R Nagareddy; Maria B Grant Journal: Cells Date: 2020-02-18 Impact factor: 6.600